Reference - Detail
| RRC ID | 43575 |
|---|---|
| Author | Chaisowwong W, Kusumoto A, Hashimoto M, Harada T, Maklon K, Kawamoto K. |
| Title | Physiological characterization of Campylobacter jejuni under cold stresses conditions: its potential for public threat. |
| Journal | J Vet Med Sci |
| Abstract |
Campylobacter jejuni is the major cause of human gastroenteritis worldwide. Under stress conditions, C. jejuni can enter a viable but non-culturable (VBNC) state. We found that the C. jejuni was able to enter a VBNC state by prolonged incubation at 4°C. The standard isolation methods using pre-enrichment steps in Bolton broth or Preston broth could not detect the VBNC cells in spiked chicken meat. The transcription levels of virulence-associated genes (flaA, flaB, cadF, ciaB, cdtA, cdtB and cdtC) were expressed in VBNC cells but in low levels. The VBNC cells retained the ability to invade Caco-2 human intestinal epithelial cells in vitro. In most cases, VBNC cells failed to resuscitate in Caco-2 cells, but in some experiments, they formed colonies after co-incubation with host cells. Collectively, C. jejuni enters into a VBNC state at 4°C and the VBNC C. jejuni remains virulent which may possibly lead to disease in humans. C. jejuni in VBNC state is a potential concern for food safety. |
| Volume | 74(1) |
| Pages | 43-50 |
| Published | 2012-1-1 |
| DOI | 10.1292/jvms.11-0305 |
| PII | JST.JSTAGE/jvms/11-0305 |
| PMID | 21891974 |
| MeSH | Animals Bacteriological Techniques Caco-2 Cells Campylobacter jejuni / metabolism* Campylobacter jejuni / pathogenicity Chickens Coculture Techniques Cold Temperature* Food Microbiology Gene Expression Regulation, Bacterial / physiology* Humans Meat / microbiology* Stress, Physiological / physiology* Virulence Virulence Factors / genetics Virulence Factors / metabolism |
| IF | 1.049 |
| Times Cited | 43 |
| WOS Category | VETERINARY SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CACO-2(RCB0988) |