論文 - 詳細
| RRC ID | 43603 |
|---|---|
| 著者 | Ueda Y, Fujita S, Nishigaki T, Arima Y, Iwata H. |
| タイトル | Substrates for human pluripotent stem cell cultures in conditioned medium of mesenchymal stem cells. |
| ジャーナル | J Biomater Sci Polym Ed |
| Abstract |
We aimed to establish a culture system of human pluripotent stem cells (hPSCs), such as human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), free from xenogeneic proteins, Matrigel(™) and conditioned medium of mouse embryonic fibroblasts. The conditioned culture medium consisted of mesenchymal stem cells derived from human bone marrow. We examined surface properties suitable for hPSC culture by using self-assembled monolayers (SAMs) of alkanethiols with four different functional groups: CH(3), OH, COOH and NH(2). hPSCs neither adhered nor proliferated on surfaces with a water contact angle higher than 40°. Based on this finding, the contact angle of a polystyrene (PSt) culture dish was reduced to less than 40°, and COOH and OH groups were introduced to its surface by oxygen plasma treatment, making the PSt dish suitable for hPSC culture. This combination of a PSt dish treated with oxygen plasma treatment and conditioned medium of mesenchymal stem cells achieved a long-term maintenance of hPSCs without differentiation. |
| 巻・号 | 23(1-4) |
| ページ | 153-65 |
| 公開日 | 2012-1-1 |
| DOI | 10.1163/092050610X545797 |
| PMID | 22133351 |
| MeSH | Animals Cell Adhesion Cell Culture Techniques / methods* Cell Proliferation Collagen / isolation & purification Culture Media, Conditioned / chemistry* Drug Combinations Fibroblasts / cytology Humans Hydrophobic and Hydrophilic Interactions Laminin / isolation & purification Mesenchymal Stem Cells / cytology* Mice Plasma Gases / chemistry Pluripotent Stem Cells / cytology* Proteoglycans / isolation & purification Sulfhydryl Compounds / chemistry Surface Properties Time Factors |
| IF | 2.69 |
| 引用数 | 1 |
| WOS 分野 | ENGINEERING, BIOMEDICAL POLYMER SCIENCE MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |