RRC ID |
43605
|
著者 |
Radzi R, Osaki T, Tsuka T, Imagawa T, Minami S, Nakayama Y, Okamoto Y.
|
タイトル |
Photodynamic hyperthermal therapy with indocyanine green (ICG) induces apoptosis and cell cycle arrest in B16F10 murine melanoma cells.
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ジャーナル |
J Vet Med Sci
|
Abstract |
We examined the effects of photodynamic hyperthemal therapy (PHT), which is a combination of photodynamic therapy (PDT) and hyperthermia (HT), on the apoptosis and cell cycle progression of murine melanoma B16F10 cells. The percentage of apoptotic cell was determined by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (PI) double staining. The cell cycle analysis was performed by PI staining with flow cytometry. The expression of cyclins and heat shock protein 70 (Hsp70) were examined by a Western blotting analysis. PHT induces death in B16F10 cells, and PHT-mediated apoptosis occurred acutely and persistently in vitro. Our study demonstrated that PHT using indocyanine green (ICG) and near infrared (NIR) light source induces apoptosis and G0/G1 cell cycle arrest in the B16F10 cells.
|
巻・号 |
74(5)
|
ページ |
545-51
|
公開日 |
2012-5-1
|
DOI |
10.1292/jvms.11-0464
|
PII |
JST.JSTAGE/jvms/11-0464
|
PMID |
22146339
|
MeSH |
Animals
Apoptosis*
Cell Cycle Checkpoints / radiation effects*
Cell Line, Tumor
Cell Survival / drug effects
Hot Temperature*
Indocyanine Green / pharmacology*
Melanoma / therapy*
Mice
Photochemotherapy*
Photosensitizing Agents / pharmacology
|
IF |
1.049
|
引用数 |
24
|
WOS 分野
|
VETERINARY SCIENCES
|
リソース情報 |
ヒト・動物細胞 |
B16F10(RCB2630) |