RRC ID 43605
著者 Radzi R, Osaki T, Tsuka T, Imagawa T, Minami S, Nakayama Y, Okamoto Y.
タイトル Photodynamic hyperthermal therapy with indocyanine green (ICG) induces apoptosis and cell cycle arrest in B16F10 murine melanoma cells.
ジャーナル J Vet Med Sci
Abstract We examined the effects of photodynamic hyperthemal therapy (PHT), which is a combination of photodynamic therapy (PDT) and hyperthermia (HT), on the apoptosis and cell cycle progression of murine melanoma B16F10 cells. The percentage of apoptotic cell was determined by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (PI) double staining. The cell cycle analysis was performed by PI staining with flow cytometry. The expression of cyclins and heat shock protein 70 (Hsp70) were examined by a Western blotting analysis. PHT induces death in B16F10 cells, and PHT-mediated apoptosis occurred acutely and persistently in vitro. Our study demonstrated that PHT using indocyanine green (ICG) and near infrared (NIR) light source induces apoptosis and G0/G1 cell cycle arrest in the B16F10 cells.
巻・号 74(5)
ページ 545-51
公開日 2012-5-1
DOI 10.1292/jvms.11-0464
PII JST.JSTAGE/jvms/11-0464
PMID 22146339
MeSH Animals Apoptosis* Cell Cycle Checkpoints / radiation effects* Cell Line, Tumor Cell Survival / drug effects Hot Temperature* Indocyanine Green / pharmacology* Melanoma / therapy* Mice Photochemotherapy* Photosensitizing Agents / pharmacology
IF 1.049
引用数 24
WOS 分野 VETERINARY SCIENCES
リソース情報
ヒト・動物細胞 B16F10(RCB2630)