論文 - 詳細
| RRC ID | 43610 |
|---|---|
| 著者 | Okamoto K, Kinoshita H, Parquet MDC, Raekiansyah M, Kimura D, Yui K, Islam MA, Hasebe F, Morita K. |
| タイトル | Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor. |
| ジャーナル | J Gen Virol |
| Abstract |
Dengue virus (DENV) causes fever and severe haemorrhagic symptoms in humans. The DEN2 16681 strain, derived from a dengue haemorrhagic fever patient, has been widely used in studies related to DENV pathogenesis, such as mouse and non-human primate haemorrhagic models and human vascular endothelial-cell permeability. To clarify the entry mechanism of the 16681 strain, we characterized a novel cell receptor for this strain. Our two major findings were as follows: firstly, the SDC2 membrane protein was an effective DEN2 16681 receptor in a cloned K562 cell line. Secondly, a heparan sulfate (HS) glycochain (of four glycochains in SDC2) is the specific binding site of DENV and seems to be involved in tissue-culture adaptation. Our findings present an entry mechanism that could be implicated for DENV adaptation and HS-mediated DENV infection. |
| 巻・号 | 93(Pt 4) |
| ページ | 761-770 |
| 公開日 | 2012-4-1 |
| DOI | 10.1099/vir.0.037853-0 |
| PMID | 22170634 |
| MeSH | Animals Chlorocebus aethiops Dengue Virus / metabolism Dengue Virus / physiology* Disease Susceptibility / virology Gene Expression Gene Silencing Heparitin Sulfate / metabolism Humans K562 Cells / virology Receptors, Virus / metabolism* Severe Dengue / metabolism Severe Dengue / virology* Syndecan-2 / metabolism* Vero Cells Virus Attachment Virus Internalization |
| IF | 3.376 |
| 引用数 | 32 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) |