| RRC ID |
43610
|
| 著者 |
Okamoto K, Kinoshita H, Parquet MDC, Raekiansyah M, Kimura D, Yui K, Islam MA, Hasebe F, Morita K.
|
| タイトル |
Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor.
|
| ジャーナル |
J Gen Virol
|
| Abstract |
Dengue virus (DENV) causes fever and severe haemorrhagic symptoms in humans. The DEN2 16681 strain, derived from a dengue haemorrhagic fever patient, has been widely used in studies related to DENV pathogenesis, such as mouse and non-human primate haemorrhagic models and human vascular endothelial-cell permeability. To clarify the entry mechanism of the 16681 strain, we characterized a novel cell receptor for this strain. Our two major findings were as follows: firstly, the SDC2 membrane protein was an effective DEN2 16681 receptor in a cloned K562 cell line. Secondly, a heparan sulfate (HS) glycochain (of four glycochains in SDC2) is the specific binding site of DENV and seems to be involved in tissue-culture adaptation. Our findings present an entry mechanism that could be implicated for DENV adaptation and HS-mediated DENV infection.
|
| 巻・号 |
93(Pt 4)
|
| ページ |
761-770
|
| 公開日 |
2012-4-1
|
| DOI |
10.1099/vir.0.037853-0
|
| PMID |
22170634
|
| MeSH |
Animals
Chlorocebus aethiops
Dengue Virus / metabolism
Dengue Virus / physiology*
Disease Susceptibility / virology
Gene Expression
Gene Silencing
Heparitin Sulfate / metabolism
Humans
K562 Cells / virology
Receptors, Virus / metabolism*
Severe Dengue / metabolism
Severe Dengue / virology*
Syndecan-2 / metabolism*
Vero Cells
Virus Attachment
Virus Internalization
|
| IF |
3.376
|
| 引用数 |
32
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
VIROLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
K562(RCB0027) |
