論文 - 詳細
| RRC ID | 43654 |
|---|---|
| 著者 | Ohno M, Ikenaka Y, Ishizuka M. |
| タイトル | Sudan III dye strongly induces CYP1A1 mRNA expression in HepG2 cells. |
| ジャーナル | J Biochem Mol Toxicol |
| Abstract |
Sudan dyes possess a high affinity to the aryl hydrocarbon receptor (AHR) and potently induce its target genes, such as cytochrome P450 (CYP) 1A1, through unknown mechanisms. We investigated a detailed event occurring in cells after binding of Sudan dye to AHR in HepG2 cells. Treatment with 10 µM Sudan III caused rapid translocation of AHR into the nucleus and increased expression levels of human CYP1A1 mRNA by approximately 20-fold after 16 and 24 h. The transactivation was due to the activation of a region located at -1137 to +59 bp from CYP1A1, in particular, four xenobiotic responsive elements (XREs) existing in the region. AHR and the Ah receptor nuclear translocator interacted with XRE sequences in a gel shift assay using nuclear extract from Sudan III--treated HepG2 cells. Moreover, we suggest that constitutive androstane receptor could modify CYP1A1 transactivation by Sudan III. |
| 巻・号 | 26(1) |
| ページ | 16-22 |
| 公開日 | 2012-1-1 |
| DOI | 10.1002/jbt.20408 |
| PMID | 22287322 |
| MeSH | Active Transport, Cell Nucleus / drug effects Aryl Hydrocarbon Receptor Nuclear Translocator / metabolism Azo Compounds / pharmacology* Coloring Agents / pharmacology* Constitutive Androstane Receptor Cytochrome P-450 CYP1A1 / genetics* Cytochrome P-450 CYP1A1 / metabolism Enzyme Induction / drug effects Hep G2 Cells Humans Protein Binding RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, Aryl Hydrocarbon / metabolism Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / metabolism Response Elements Transcription, Genetic / drug effects* Transcriptional Activation / drug effects |
| IF | 3.606 |
| 引用数 | 7 |
| WOS 分野 | TOXICOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |