Reference - Detail
| RRC ID | 43670 |
|---|---|
| Author | Takeshita H, Kitano M, Iwasaki T, Kitano S, Tsunemi S, Sato C, Sekiguchi M, Azuma N, Miyazawa K, Hla T, Sano H. |
| Title | Sphingosine 1-phosphate (S1P)/S1P receptor 1 signaling regulates receptor activator of NF-κB ligand (RANKL) expression in rheumatoid arthritis. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Sphingosine 1-phosphate (S1P)/S1P receptor 1 (S1P1) signaling plays an important role in synovial cell proliferation and inflammatory gene expression by rheumatoid arthritis (RA) synoviocytes. The purpose of this study is to clarify the role of S1P/S1P1 signaling in the expression of receptor activator of NF-κB ligand (RANKL) in RA synoviocytes and CD4(+) T cells. We demonstrated MH7A cells, a human RA synovial cell line, and CD4(+) T cells expressed S1P1 and RANKL. Surprisingly, S1P increased RANKL expression in MH7A cells and CD4(+) T cells in a dose-dependent manner. Moreover, S1P enhanced RANKL expression induced by stimulation with TNF-α in MH7A cells and CD4(+) T cells. These effects of S1P in MH7A cells were inhibited by pretreatment with PTX, a specific Gi/Go inhibitor. These findings suggest that S1P/S1P1 signaling may play an important role in RANKL expression by MH7A cells and CD4(+) T cells. S1P/S1P1 signaling of RA synoviocytes is closely connected with synovial hyperplasia, inflammation, and RANKL-induced osteoclastogenesis in RA. Thus, regulation of S1P/S1P1 signaling may become a novel therapeutic target for RA. |
| Volume | 419(2) |
| Pages | 154-9 |
| Published | 2012-3-9 |
| DOI | 10.1016/j.bbrc.2012.01.103 |
| PII | S0006-291X(12)00150-7 |
| PMID | 22326262 |
| PMC | PMC3857967 |
| MeSH | Arthritis, Rheumatoid / immunology* CD4-Positive T-Lymphocytes / immunology Cells, Cultured GTP-Binding Protein alpha Subunits, Gi-Go / metabolism Humans Lysophospholipids / metabolism* RANK Ligand / biosynthesis* RNA, Messenger / biosynthesis RNA, Messenger / genetics Receptors, Lysosphingolipid / metabolism* Signal Transduction Sphingosine / analogs & derivatives* Sphingosine / metabolism Synovial Fluid / immunology |
| IF | 2.985 |
| Times Cited | 24 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MH7A(RCB1512) |