Reference - Detail
RRC ID | 43672 |
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Author | Watanabe M, Ohno S, Nakajin S. |
Title | Effects of bisphenol A on the expression of cytochrome P450 aromatase (CYP19) in human fetal osteoblastic and granulosa cell-like cell lines. |
Journal | Toxicol Lett |
Abstract |
The effects of bisphenol A (BPA), an endocrine disruptor, on aromatase (CYP19) expression in human osteoblastic (SV-HFO) and ovarian granulosa-like (KGN) cell lines were examined. CYP19 enzyme activity was suppressed in the presence of BPA in a dose-dependent fashion in both cell lines. CYP19 gene transcript expression, as well as activities of promoter I.4 in SV-HFO and promoter II in KGN, was down-regulated by BPA, suggesting that BPA affects CYP19 at the gene-expression level. These data and the previous finding that BPA induced the down-regulation of promoter I.1 activity within the human placental cell line suggest that there may be a conserved signaling pathway that down-regulates CYP19 expression in response to BPA in both cell lines. Additionally, differences between promoter I.4 and II suggest that there may be cell- and promoter-specific down-regulating mechanisms downstream from the actions of BPA. |
Volume | 210(1) |
Pages | 95-9 |
Published | 2012-4-5 |
DOI | 10.1016/j.toxlet.2012.01.020 |
PII | S0378-4274(12)00045-8 |
PMID | 22327052 |
MeSH | Aromatase / biosynthesis* Benzhydryl Compounds Cell Line Dose-Response Relationship, Drug Down-Regulation / drug effects Female Fetus / drug effects Fetus / enzymology Gene Expression / drug effects Granulosa Cells / drug effects* Granulosa Cells / enzymology Humans Osteoblasts / drug effects* Osteoblasts / enzymology Phenols / pharmacology* Promoter Regions, Genetic / drug effects Reverse Transcriptase Polymerase Chain Reaction |
IF | 3.569 |
Times Cited | 13 |
WOS Category | TOXICOLOGY |
Resource | |
Human and Animal Cells | KGN(RCB1154) |