RRC ID 4369
Author Tanaka K, Shirakawa H, Okada K, Konno M, Nakagawa T, Serikawa T, Kaneko S.
Title Increased Ca2+ channel currents in cerebellar Purkinje cells of the ataxic groggy rat.
Journal Neurosci. Lett.
Abstract The ataxic groggy rat (strain name; GRY) is an autosomal recessive neurological mutant found in a closed colony of Slc:Wistar rats. Recent genetic analysis has identified the missense (M251K) mutation in the alpha(1) subunit of the Ca(V)2.1 (P/Q-type) voltage-dependent Ca(2+) channel gene (Cacna1a) of GRY rat. In this study, we found that high-voltage-activated (HVA) Ca(2+) channel currents in acutely dissociated Purkinje cells of GRY rats showed increased (not decreased) current density and depolarizing shift of the activation and inactivation curves compared with those of normal Wistar rats. In contrast low-voltage-activated (LVA) Ca(2+) channel currents of GRY rats showed no significant changes. These results suggest that functional alteration of Ca(2+) channel currents in cerebellar Purkinje cells of GRY rats is attributed to the change of HVA Ca(2+) channel currents, and that increased HVA Ca(2+) channel function underlies the cerebellar dysfunction and ataxic phenotype of GRY rats.
Volume 426(2)
Pages 75-80
Published 2007-10-16
DOI 10.1016/j.neulet.2007.08.046
PII S0304-3940(07)00934-2
PMID 17884288
MeSH Animals Animals, Newborn Calcium Channels, N-Type / physiology* Cerebellar Ataxia / genetics Cerebellar Ataxia / pathology* Cerebellum / pathology* Dose-Response Relationship, Radiation Electric Stimulation / methods Membrane Potentials / drug effects Membrane Potentials / genetics Membrane Potentials / physiology* Patch-Clamp Techniques / methods Purkinje Cells / physiology* Rats Rats, Wistar
IF 2.173
Times Cited 2
Rats GRY/Idr(strainID=498)