Reference - Detail
| RRC ID | 43705 |
|---|---|
| Author | Sato F, Sato H, Jin D, Bhawal UK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H. |
| Title | Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Smads are intracellular signaling mediators. Complexes of Smad2 and Smad3 with Smad4 transmit transforming growth factor-beta (TGF-β) receptor-induced signaling. Snail plays important roles in mesoderm formation, gastrulation, neural crest development, and epithelial mesenchymal transition. However, it remains unknown whether Smad3 and Snail expression is circadian rhythm-dependent. Here, we showed for the first time that Smad3 and Snail show circadian expression in human gingival fibroblasts (HGF-1) and human mesenchymal stem cells (MSC) after serum shock. They also showed circadian expression in the mouse liver. We confirmed that BMAL1/2, DEC1/2, VEGF, and PER1/2/3 also show circadian expression in both HGF-1 and MSC. The mRNA peaks and phases in circadian expression of these genes differed between HGF-1 and MSC. In a luciferase assay, Smad3 promoter activity was upregulated by CLOCK/BMAL1. These findings suggest that Smad3 and Snail have circadian rhythm in vitro and vivo, and that circadian expression of Smad3 depends on CLOCK/BMAL1. |
| Volume | 419(2) |
| Pages | 441-6 |
| Published | 2012-3-9 |
| DOI | 10.1016/j.bbrc.2012.02.076 |
| PII | S0006-291X(12)00309-9 |
| PMID | 22382019 |
| MeSH | ARNTL Transcription Factors / biosynthesis Animals Basic Helix-Loop-Helix Transcription Factors / biosynthesis CLOCK Proteins / metabolism Cells, Cultured Circadian Rhythm* Fibroblasts / metabolism* Gingiva / metabolism* Humans Liver / metabolism* Male Mesenchymal Stem Cells / metabolism* Mice Period Circadian Proteins / biosynthesis Smad3 Protein / biosynthesis* Snail Family Transcription Factors Transcription Factors / biosynthesis* Tumor Suppressor Proteins / biosynthesis Vascular Endothelial Growth Factor A / biosynthesis |
| IF | 2.985 |
| Times Cited | 17 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MSC-R37(MSC; MSC-R37) |