RRC ID 4372
Author Shono S, Kose H, Yamada T, Matsumoto K.
Title Proteomic analysis of a diabetic congenic rat identified age-dependent alteration of an acidic protein.
Journal J. Med. Invest.
Abstract Proteomic analysis was performed in an attempt to identify a gene responsible for the expression of type 2 diabetes using a congenic rat, F.O-Nidd2/of, which possesses a single hyperglycemic QTL locus derived from the diabetic OLETF (Otsuka Long-Evans Tokushima Fatty) rat. Since the genetic difference between the congenic and its host strain, the F344 rat, is limited to the introgressed segment of 38 cM or ca. 2% of the rat whole genome, any discordant protein spots on two dimensional polyacrylamid gel electrophoresis (2D PAGE) will be considered strong candidate genes of this locus. Here we analyzed ca. one thousand protein spots in three different tissue types, liver, muscle and pancreas at 10, 20 and 30 weeks of age, we found that an acidic protein of 55 kD in muscle tissue shifts towards acidic end in an age dependent fashion in the congenic strain. However, the shift was not observed in the control rat, which is intriguing because the timing of the shift corresponds to the age at which hyperglycemia begins in the congenic. Future biochemical analysis should aid in elucidating the molecular mechanisms of glucose metabolism.
Volume 54(3-4)
Pages 289-94
Published 2007-8
PII JST.JSTAGE/jmi/54.289
PMID 17878678
MeSH Aging / metabolism Animals Animals, Congenic Diabetes Mellitus, Type 2 / genetics Diabetes Mellitus, Type 2 / metabolism* Electrophoresis, Gel, Two-Dimensional Hydrogen-Ion Concentration Male Molecular Weight Muscle Proteins / chemistry Muscle Proteins / metabolism* Proteomics Quantitative Trait Loci Rats Rats, Inbred F344 Rats, Inbred OLETF
Rats F344.OLETF-(D14Rat23-D14Rat12)/Tj(strainID=87)