論文 - 詳細
| RRC ID | 43724 |
|---|---|
| 著者 | Shimada T, Takai Y, Shinohara K, Yamasaki A, Tominaga-Yoshino K, Ogura A, Toi A, Asano K, Shintani N, Hayata-Takano A, Baba A, Hashimoto H. |
| タイトル | A simplified method to generate serotonergic neurons from mouse embryonic stem and induced pluripotent stem cells. |
| ジャーナル | J Neurochem |
| Abstract |
We have developed a new simple method to induce serotonergic neurons from embryonic stem (ES) and induced pluripotent stem cells. When ES or induced pluripotent stem cells were cultured on a thick gel layer of Matrigel, most colonies extended TuJ1-positive neurites. We found that noggin, a known antagonist of bone morphogenic protein, induces ES cells to express genes involved in serotonergic differentiation, such as Nkx2.2, Pet-1, Sonic hedgehog, tryptophan hydroxylase 2, and serotonin transporter, as well as increases high potassium-induced release of serotonin. To concentrate serotonergic neurons, ES cells carrying Pet-1-enhancer-driven enhanced green fluorescent protein were differentiated and sorted into about 80% pure cultures of serotonergic neurons. Whole cell voltage-clamp recordings showed a voltage-dependent current in dissociated neurons. This simplified method provides an alternative option for serotonergic differentiation of pluripotent stem cells and will likely contribute a deeper understanding regarding the nature of serotonergic neurons and open new therapeutic perspectives for the treatment of psychiatric disorders. |
| 巻・号 | 122(1) |
| ページ | 81-93 |
| 公開日 | 2012-7-1 |
| DOI | 10.1111/j.1471-4159.2012.07724.x |
| PMID | 22404309 |
| MeSH | Animals Bone Morphogenetic Protein 4 / pharmacology Carrier Proteins / metabolism Carrier Proteins / pharmacology Cell Differentiation / physiology Cell Line Collagen / metabolism Dose-Response Relationship, Drug Drug Combinations Electric Stimulation Embryo, Mammalian Embryonic Stem Cells / physiology* Flow Cytometry Green Fluorescent Proteins / genetics Hippocampus / cytology Homeobox Protein Nkx-2.2 Induced Pluripotent Stem Cells / physiology* Laminin / metabolism Mice Organ Culture Techniques Proteins / genetics Proteins / metabolism Proteoglycans / metabolism RNA, Messenger / metabolism RNA, Untranslated Serotonergic Neurons / physiology* Serotonin / metabolism Transcription Factors / genetics Transduction, Genetic Tubulin / metabolism Tyrosine 3-Monooxygenase / metabolism |
| IF | 4.066 |
| 引用数 | 21 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-G2/PA6(RCB1127) |