RRC ID 43730
Author Ushida H, Chano T, Minami K, Kita H, Kawakami T, Okabe H, Okada Y, Okamoto K.
Title Therapeutic potential of SOX2 inhibition for embryonal carcinoma.
Journal J Urol
Abstract PURPOSE:Some nonseminomatous germ cell tumors are resistant to any type of chemotherapy. Control of embryonal carcinoma cells is crucial to manage nonseminomatous germ cell tumors. We established SOX2 targeting therapy in an embryonal carcinoma model.
MATERIALS AND METHODS:SOX2 expression was evaluated in a series of testicular germ cell tumor tissue samples. The antitumor effect of SOX2 knockdown was analyzed in vitro and in vivo using an embryonal carcinoma model.
RESULTS:In testicular germ cell tumor tissue SOX2 was expressed in the foci of embryonal carcinoma but negative in seminoma and yolk sac tumors. In an embryonal carcinoma model SOX2-siRNA induced apoptotic cell death in vitro and significant growth suppression in vivo.
CONCLUSIONS:This study shows the therapeutic potential of SOX2 silencing for embryonal carcinoma. However, further improvements are needed in SOX2-siRNA delivery to the tumor.
Volume 187(5)
Pages 1876-81
Published 2012-5-1
DOI 10.1016/j.juro.2011.12.058
PII S0022-5347(11)06005-8
PMID 22425046
MeSH Animals Carcinoma, Embryonal / metabolism* Carcinoma, Embryonal / pathology Carcinoma, Embryonal / therapy* Cell Death Cell Line, Tumor Disease Models, Animal Gene Silencing Immunohistochemistry Male Mice Mice, Inbred Strains RNA, Small Interfering / therapeutic use SOXB1 Transcription Factors / antagonists & inhibitors* SOXB1 Transcription Factors / metabolism* Seminoma / metabolism Seminoma / pathology Testicular Neoplasms / metabolism* Testicular Neoplasms / pathology Testicular Neoplasms / therapy* Transfection
IF 5.925
Times Cited 8
Human and Animal Cells NEC8(RCB0489)