論文 - 詳細
| RRC ID | 43738 |
|---|---|
| 著者 | Hamano R, Miyata H, Yamasaki M, Sugimura K, Tanaka K, Kurokawa Y, Nakajima K, Takiguchi S, Fujiwara Y, Mori M, Doki Y. |
| タイトル | High expression of Lin28 is associated with tumour aggressiveness and poor prognosis of patients in oesophagus cancer. |
| ジャーナル | Br J Cancer |
| Abstract |
BACKGROUND:Lin28 is a negative regulator of the tumour suppressor microRNA, let-7, suggesting its role in tumourigenesis. However, the clinical significance of Lin28 expression in oesophageal cancer has not been elucidated. METHODS:Lin28 and Lin28B expression was examined by immunohistochemistry in 161 tissues from patients with oesophageal cancer who had undergone curative surgery. The relationship between the expressions of Lin28 and Lin28B and various clinicopathological factors was examined. In vitro assays were conducted to determine the role of Lin28 in aggressiveness of oesophageal cancers using oesophageal cancer cell line. RESULTS:Lin28 and Lin28B were overexpressed in oesophageal cancer cells compared with non-cancerous epithelial cells, especially in the invasive front. High expression of Lin28 and Lin28B correlated significantly with lymph node metastasis and poor prognosis. High expression of Lin28B expression correlated significantly with low expression of let-7. Multivariate analysis also identified Lin28B expression as an independent prognostic factor. In vitro assays showed that the proliferative and invasive activities were significantly reduced in Lin28B-knockdown cells, compared with control cells. CONCLUSION:High expression of Lin28 is associated with poor prognosis and high tumour aggressiveness in oesophageal cancer and these effects are mediated through increased proliferation and invasiveness of oesophageal cancer cells. |
| 巻・号 | 106(8) |
| ページ | 1415-23 |
| 公開日 | 2012-4-10 |
| DOI | 10.1038/bjc.2012.90 |
| PII | bjc201290 |
| PMID | 22433967 |
| PMC | PMC3326671 |
| MeSH | Cell Proliferation DNA-Binding Proteins / biosynthesis DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Esophageal Neoplasms / diagnosis Esophageal Neoplasms / genetics Esophageal Neoplasms / metabolism* Esophageal Neoplasms / pathology* Female Humans Immunohistochemistry Male Middle Aged Prognosis RNA-Binding Proteins / biosynthesis RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Real-Time Polymerase Chain Reaction |
| IF | 5.791 |
| 引用数 | 58 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | TE-1(RCB1894) TE-8(RCB2098) TE-10(RCB2099) TE-15(RCB1951) |