Reference - Detail
RRC ID | 43739 |
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Author | Cavet ME, Harrington KL, VanDerMeid KR, Ward KW, Zhang JZ. |
Title | In vitro biocompatibility assessment of multipurpose contact lens solutions: effects on human corneal epithelial viability and barrier function. |
Journal | Cont Lens Anterior Eye |
Abstract |
PURPOSE:To explore the in vitro effects of multipurpose contact lens solutions (MPSs) on corneal epithelial barrier function and viability. METHODS:Human corneal epithelial cells (HCEpiC) were exposed to 50% MPSs A-G. Viability was determined using metabolic activity, protease release and caspase assays. Barrier function was evaluated using immunostaining for the tight junction protein zonnula occludens-1 (ZO-1) and resistance measurements. RESULTS:MPS A and G did not affect HCEpiC monolayer viability after 2 h, while MPSs B-F significantly decreased viability. There was a significant decrease in stratified HCEpiC viability after exposure to MPSs B-E for 2 h, while there was no effect of MPS A. After exposure of HCEpiC monolayers to MPS A, F or G for 30 min, ZO-1 staining appeared similar to control. HCEpiC exposed to MPSs B and C demonstrated tight junction breakdown. There was no significant change in HCEpiC monolayer resistance after exposure to MPS A or F for 2 h, while MPSs B-E and G reduced resistance. After exposure to MPS A-E, stratified HCEpiC resistance was significantly decreased after 2 or 4 h. The decrease in resistance was significantly less with MPS A as compared to the other MPSs. CONCLUSIONS:MPSs caused varying modifications to cell viability and barrier function in monolayer and stratified HCEpiC. MPS A did not alter monolayer HCEpiC viability or barrier function, while MPSs B-G caused significant decreases of at least one parameter. Furthermore, MPS A had significantly less effect than MPSs B-E on viability and barrier function of stratified HCEpiC. |
Volume | 35(4) |
Pages | 163-70 |
Published | 2012-8-1 |
DOI | 10.1016/j.clae.2012.02.003 |
PII | S1367-0484(12)00030-6 |
PMID | 22436848 |
MeSH | Biocompatible Materials / pharmacology* Cell Membrane Permeability / drug effects Cell Survival / drug effects Cells, Cultured Contact Lens Solutions / pharmacology* Electric Impedance Epithelium, Corneal / cytology Epithelium, Corneal / drug effects* Epithelium, Corneal / metabolism Fluorescent Antibody Technique, Indirect Humans Materials Testing Zonula Occludens-1 Protein / metabolism |
IF | 2.578 |
Times Cited | 9 |
WOS Category | OPHTHALMOLOGY |
Resource | |
Human and Animal Cells | HCE-T(RCB2280) |