RRC ID 43764
著者 Kondo Y, Ishitsuka Y, Kadowaki D, Kuroda M, Tanaka Y, Nagatome M, Irikura M, Hirata S, Sato K, Maruyama T, Hamasaki N, Irie T.
タイトル Phosphoenolpyruvic acid, an intermediary metabolite of glycolysis, as a potential cytoprotectant and anti-oxidant in HeLa cells.
ジャーナル Biol Pharm Bull
Abstract This study examined the cytoprotective and anti-oxidative properties of phosphoenolpyruvic acid (PEP), a glycolysis metabolite with a high-energy phosphate group. PEP (0.1-10 mM) significantly attenuated the decrease in cell viability induced by hydrogen peroxide (H(2)O(2)) in HeLa cells in a dose-dependent manner. PEP also inhibited the decrease in calcein-acetomethoxy-stained cells and the increase in propidium iodide-stained cells that were induced by H(2)O(2). The H(2)O(2)-stimulated increase in intracellular reactive oxygen species was significantly reduced by PEP. PEP also demonstrated scavenging potential against hydroxyl radicals, as assessed by the electron paramagnetic resonance method. In addition, PEP demonstrated scavenging potential against the 1,1-diphenyl-2-picrylhydrazyl radical, a representative artificial radical, although the potential is very weak. PEP (10 mM) slightly inhibited the decrease in cellular ATP content induced by H(2)O(2), but did not show any effects at low doses (0.1, 1 mM). PEP (0.1-10 mM) also attenuated the cell injury but not the decrease in intracellular ATP content, induced by 2-deoxy-D-glucose, a glycolysis inhibitor. These results indicate that PEP exerts cytoprotective effects and has anti-oxidative potential, although the precise cytoprotective mechanisms are not fully elucidated. We suggest that PEP is a functional carbohydrate metabolite with cytoprotective and anti-oxidative activity, and is potentially useful as a therapeutic agent against diseases that involve the oxidative stress.
巻・号 35(4)
ページ 606-11
公開日 2012-1-1
DOI 10.1248/bpb.35.606
PII JST.JSTAGE/bpb/35.606
PMID 22466568
MeSH Adenosine Triphosphate / antagonists & inhibitors Adenosine Triphosphate / metabolism Antioxidants / pharmacology* Cell Survival / drug effects Cytoprotection / drug effects* Deoxyglucose / pharmacology Glycolysis HeLa Cells Humans Hydrogen Peroxide / pharmacology Oxidants / pharmacology Phosphoenolpyruvate / analogs & derivatives* Phosphoenolpyruvate / pharmacology
IF 1.863
引用数 3
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 HeLa(RCB0007)