RRC ID 43791
Author Yamamoto N, Maeda H, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Kono K, Koori K, Teramatsu Y, Akamine A.
Title Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells.
Journal J Clin Periodontol
Abstract AIM:To investigate Glial cell line-derived neurotrophic factor (GDNF) expression in normal and wounded rat periodontal ligament (PDL) and the effects of GDNF on human PDL cells (HPDLCs) migration and extracellular matrix expression in HPDLCs.
MATERIAL AND METHODS:The expression of GDNF and GDNF receptors was examined by immunocyto/histochemical analyses. Gene expression in HPDLCs treated with GDNF, interleukin-1 beta (IL-1β), or tumour necrosis factor-alpha (TNF-α) was quantified by quantitative RT-PCR (qRT-PCR). In addition, we examined the migratory effect of GDNF on HPDLCs.
RESULTS:GDNF was expressed in normal rat PDL and cultured HPDLCs. HPDLCs also expressed GDNF receptors. In wounded rat PDL, GDNF expression was up-regulated. QRT-PCR analysis revealed that IL-1β and TNF-α significantly increased the expression of GDNF in HPDLCs. Furthermore, GDNF induced migration of HPDLCs, which was blocked by pre-treatment with the peptide including Arg-Gly-Asp (RGD) sequence, or neutralizing antibodies against integrin αVβ3 or GDNF. Also, GDNF up-regulated expression of bone sialoprotein (BSP) and fibronectin in HPDLCs.
CONCLUSIONS:GDNF expression is increased in rat wounded PDL tissue and HPDLCs treated with pro-inflammatory cytokines. GDNF enhances the expression of BSP and fibronectin, and migration in an RGD-dependent manner via the integrin αVβ3. These findings suggest that GDNF may contribute to wound healing in PDL tissue.
Volume 39(6)
Pages 556-64
Published 2012-6-1
DOI 10.1111/j.1600-051X.2012.01881.x
PMID 22512503
MeSH Adult Alveolar Bone Loss / metabolism* Animals Cell Adhesion Cell Movement / drug effects Cells, Cultured Extracellular Matrix Proteins / biosynthesis* Extracellular Matrix Proteins / genetics Female Fibronectins / biosynthesis Fibronectins / genetics Gene Expression Regulation Glial Cell Line-Derived Neurotrophic Factor / genetics Glial Cell Line-Derived Neurotrophic Factor / metabolism* Glial Cell Line-Derived Neurotrophic Factor / pharmacology Glial Cell Line-Derived Neurotrophic Factor Receptors / metabolism Humans Integrin-Binding Sialoprotein / biosynthesis Integrin-Binding Sialoprotein / genetics Interleukin-1beta / pharmacology Male Oligopeptides / metabolism Periodontal Ligament / cytology Periodontal Ligament / drug effects* Periodontal Ligament / metabolism* Periodontal Ligament / pathology Platelet Glycoprotein GPIIb-IIIa Complex / metabolism Rats Rats, Sprague-Dawley Recombinant Proteins / pharmacology Tumor Necrosis Factor-alpha / pharmacology Wound Healing / physiology Young Adult
IF 5.241
Times Cited 12
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