RRC ID 43823
Author Shimada E, Kataoka H, Miyazawa Y, Yamamoto M, Igarashi T.
Title Lipoproteins of Actinomyces viscosus induce inflammatory responses through TLR2 in human gingival epithelial cells and macrophages.
Journal Microbes Infect
Abstract Actinomyces viscosus has been suggested to be associated with periodontal disease. However, the pathogenicity of this bacterium is not known. In this study, we examined inflammation-inducing activity by A. viscosus. Whole cells and a lipophilic fraction of A. viscosus ATCC19246 induced production of interleukin-8 and tumor necrosis factor alpha from both human oral epithelial cells and human monocytoid cells. This cytokine production was blocked by lipoprotein lipase treatment of the lipophilic fraction. In addition, anti-Toll-like receptor 2 antibody blocked the cytokine production. These results suggest that lipoprotein of A. viscosus triggers inflammatory responses in periodontitis by activation of Toll-like receptor 2.
Volume 14(11)
Pages 916-21
Published 2012-9-1
DOI 10.1016/j.micinf.2012.04.015
PII S1286-4579(12)00118-9
PMID 22561467
MeSH Actinomyces viscosus / chemistry Actinomyces viscosus / immunology* Actinomycosis / immunology Actinomycosis / microbiology Analysis of Variance Bacterial Proteins / immunology Cytokines / immunology Epithelial Cells / cytology Epithelial Cells / immunology Gingiva / cytology Gingiva / immunology* Gingival Diseases / immunology Gingival Diseases / microbiology HEK293 Cells Host-Pathogen Interactions Humans Inflammation / immunology Lipoproteins / immunology* Macrophages / cytology Macrophages / immunology Toll-Like Receptor 2 / immunology*
IF 2.373
Times Cited 13
Human and Animal Cells