| RRC ID |
43826
|
| 著者 |
Sakai S, Inagaki H, Liu Y, Matsuyama T, Kihara T, Miyake J, Kawakami K, Taya M.
|
| タイトル |
Rapidly serum-degradable hydrogel templating fabrication of spherical tissues and curved tubular structures.
|
| ジャーナル |
Biotechnol Bioeng
|
| Abstract |
Development of the techniques for fabricating three-dimensional tissues still poses significant challenges for tissue engineering. We used hydrogels obtained from phenol-substituted amylopectin (AP-Ph) as templates for preparing multicellular spherical tissues (MSTs) and endothelialized curved tubular structures in type I collagen gel. AP-Ph hydrogel microparticles of diameter 200 µm and fibers of diameter 500 µm disappeared within hours of soaking in a serum-containing medium. HeLa cells and human endothelial cells were enclosed in the microparticles and hydrogel fibers, respectively, and then embedded in Ca-alginate microcapsules or the collagen gel. The enclosed cells were released in cavities formed by hydrogel degradation in the serum-containing medium. The released HeLa cells in the spherical cavities grew and formed MSTs, eventually filling the cavities. The spherical tissues were easily harvested by liquefying the Ca-alginate hydrogel microcapsule membrane by chelation using sodium citrate. The released endothelial cells grew on the tubular cavity surfaces and formed tubular structures. An endothelial cell network was formed by cell migration into the collagen gel. These results demonstrate the potential of serum-degradable AP-Ph hydrogels in constructing three-dimensional tissues.
|
| 巻・号 |
109(11)
|
| ページ |
2911-9
|
| 公開日 |
2012-11-1
|
| DOI |
10.1002/bit.24550
|
| PMID |
22566297
|
| MeSH |
Absorbable Implants
Alginates / metabolism
Amylopectin / metabolism
Cells, Cultured
Citrates / metabolism
Collagen / metabolism
Endothelial Cells
Epithelial Cells
Glucuronic Acid / metabolism
Hexuronic Acids / metabolism
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate / metabolism*
Serum / metabolism*
Sodium Citrate
Tissue Engineering / methods*
|
| IF |
4.002
|
| 引用数 |
9
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
