論文 - 詳細
| RRC ID | 43839 |
|---|---|
| 著者 | Tahara Y, Kaneko T, Toita R, Yoshiyama C, Kitaoka T, Niidome T, Katayama Y, Kamiya N, Goto M. |
| タイトル | A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells. |
| ジャーナル | J Control Release |
| Abstract |
A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells. |
| 巻・号 | 161(3) |
| ページ | 713-21 |
| 公開日 | 2012-8-10 |
| DOI | 10.1016/j.jconrel.2012.05.001 |
| PII | S0168-3659(12)00329-X |
| PMID | 22580226 |
| MeSH | Animals CHO Cells Cholesterol / chemistry Cricetinae Cricetulus Cytochromes c / administration & dosage* DNA / administration & dosage Drug Carriers / administration & dosage* Drug Carriers / chemistry Horseradish Peroxidase / administration & dosage* Nanotechnology / methods Oils / chemistry Oligopeptides / chemistry Ovalbumin / administration & dosage* Particle Size Phosphatidylcholines / chemistry Phosphatidylethanolamines / chemistry Stearates / chemistry Transfection / methods* Water / chemistry |
| IF | 7.727 |
| 引用数 | 8 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |