RRC ID 43850
Author Wright B, Cave RA, Cook JP, Khutoryanskiy VV, Mi S, Chen B, Leyland M, Connon CJ.
Title Enhanced viability of corneal epithelial cells for efficient transport/storage using a structurally modified calcium alginate hydrogel.
Journal Regen Med
Abstract AIMS:Therapeutic limbal epithelial stem cells could be managed more efficiently if clinically validated batches were transported for 'on-demand' use.
MATERIALS & METHODS:In this study, corneal epithelial cell viability in calcium alginate hydrogels was examined under cell culture, ambient and chilled conditions for up to 7 days.
RESULTS:Cell viability improved as gel internal pore size increased, and was further enhanced with modification of the gel from a mass to a thin disc. Ambient storage conditions were optimal for supporting cell viability in gel discs. Cell viability in gel discs was significantly enhanced with increases in pore size mediated by hydroxyethyl cellulose.
CONCLUSION:Our novel methodology of controlling alginate gel shape and pore size together provides a more practical and economical alternative to established corneal tissue/cell storage methods.
Volume 7(3)
Pages 295-307
Published 2012-5
DOI 10.2217/rme.12.7
PMID 22594324
MeSH Alginates / chemistry* Alginates / pharmacology* Animals Cattle Cell Differentiation / drug effects Cell Line Cell Survival / drug effects Cells, Immobilized / cytology Cells, Immobilized / drug effects Cellulose / analogs & derivatives Cellulose / pharmacology Epithelial Cells / cytology* Epithelial Cells / drug effects* Epithelium, Corneal / cytology* Glucuronic Acid / chemistry Glucuronic Acid / pharmacology Hexuronic Acids / chemistry Hexuronic Acids / pharmacology Humans Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry* Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology* Limbus Corneae / cytology Microscopy, Electron, Scanning Porosity / drug effects Preservation, Biological* Transportation*
IF 2.992
Times Cited 16
Human and Animal Cells