論文 - 詳細
| RRC ID | 43870 |
|---|---|
| 著者 | Yamawaki H, Kameshima S, Usui T, Okada M, Hara Y. |
| タイトル | A novel adipocytokine, chemerin exerts anti-inflammatory roles in human vascular endothelial cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Chemerin is a recently identified adipocytokine which plays a role on inflammation and adipocytes metabolism. However, its function in vasculature is largely unknown. We examined the effects of chemerin on vascular endothelial inflammatory states. Treatment of human umbilical vein endothelial cells with chemerin (300 ng/ml, 20 min) induced phosphorylation of Akt (Ser473) and endothelial nitric oxide (NO) synthase (eNOS) (Ser1177). Consistently, chemerin increased intracellular cyclic GMP content. Pretreatment with chemerin (1-300 ng/ml, 24 h) significantly inhibited phosphorylation of nuclear factor (NF)-κB p65 (Ser536) and p38 as well as vascular cell adhesion molecule (VCAM)-1 expression induced by tumor necrosis factor (TNF)-α (5 ng/ml, 20 min-6 h). Inhibitor of NF-κB or p38 significantly inhibited the TNF-α-induced VCAM-1 expression. Chemerin also inhibited TNF-α-induced VCAM-1 expression in rat isolated aorta. Moreover, chemerin significantly inhibited monocytes adhesion to TNF-α-stimulated endothelial cells. The inhibitory effect of chemerin on TNF-α-induced VCAM-1 was reversed by a NOS inhibitor. Conversely, an NO donor, sodium nitroprusside significantly inhibited TNF-α-induced VCAM-1. The present results for the first time demonstrate that chemerin plays anti-inflammatory roles by preventing TNF-α-induced VCAM-1 expression and monocytes adhesion in vascular endothelial cells. The effect is mediated via inhibiting activation of NF-κB and p38 through stimulation of Akt/eNOS signaling and NO production. |
| 巻・号 | 423(1) |
| ページ | 152-7 |
| 公開日 | 2012-6-22 |
| DOI | 10.1016/j.bbrc.2012.05.103 |
| PII | S0006-291X(12)00993-X |
| PMID | 22634313 |
| MeSH | Adipokines / pharmacology Adipokines / physiology* Animals Cell Adhesion / drug effects Chemokines / pharmacology Chemokines / physiology* Human Umbilical Vein Endothelial Cells / drug effects Human Umbilical Vein Endothelial Cells / metabolism* Humans Inflammation / metabolism* Intercellular Signaling Peptides and Proteins Male Monocytes / drug effects Monocytes / physiology NF-kappa B / antagonists & inhibitors NF-kappa B / metabolism Nitric Oxide / metabolism Nitric Oxide Synthase Type III / metabolism Rats Rats, Wistar Recombinant Proteins / pharmacology Tumor Necrosis Factor-alpha / pharmacology Vascular Cell Adhesion Molecule-1 / metabolism p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 2.985 |
| 引用数 | 52 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | U-937 DE-4(RCB0435) |