論文 - 詳細
| RRC ID | 43877 |
|---|---|
| 著者 | Suzuki D, Yamada A, Aizawa R, Funato S, Matsumoto T, Suzuki W, Takami M, Miyamoto Y, Suzawa T, Yamamoto M, Baba K, Kamijo R. |
| タイトル | BMP2 differentially regulates the expression of Gremlin1 and Gremlin2, the negative regulators of BMP function, during osteoblast differentiation. |
| ジャーナル | Calcif Tissue Int |
| Abstract |
Bone morphogenetic proteins (BMPs) control the expressions of many genes involved in bone formation. On the basis of our hypothesis that BMP2 stimulation-regulated gene expression plays a critical role in osteoblast differentiation, we performed genome-wide screening of messenger RNA from BMP2-treated and -untreated C2C12 cells using a DNA microarray technique. We found that the expressions of Gremlin1 and Gremlin2, which are known BMP antagonists, were bidirectionally regulated by BMP2. Gremlin1 was down-regulated by BMP2, while Gremlin2 was up-regulated in both time- and dose-dependent manners. Ablation of Gremlin1 or Gremlin2 enhanced osteoblast differentiation induced by BMP2. On the other hand, treatment with recombinant Gremlin1 inhibited BMP2-induced osteoblast differentiation. Furthermore, treatment with Smad4 siRNA and the p38 MAPK inhibitor SB203580 suppressed BMP2-induced Gremlin2 gene expression. The differential regulation of Gremlin1 and Gremlin2 gene expressions by BMP2 may explain the critical function of these genes during osteoblast differentiation. |
| 巻・号 | 91(1) |
| ページ | 88-96 |
| 公開日 | 2012-7-1 |
| DOI | 10.1007/s00223-012-9614-5 |
| PMID | 22644325 |
| MeSH | Animals Bone Morphogenetic Protein 2 / antagonists & inhibitors Bone Morphogenetic Protein 2 / genetics Bone Morphogenetic Protein 2 / metabolism* Cell Differentiation* Cells, Cultured Cytokines Gene Expression Regulation Mice Osteoblasts / cytology* Osteoblasts / metabolism Proteins / genetics* Proteins / metabolism RNA, Small Interfering / metabolism Signal Transduction Smad4 Protein / genetics Smad4 Protein / metabolism |
| IF | 3.423 |
| 引用数 | 19 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |