論文 - 詳細
| RRC ID | 43882 |
|---|---|
| 著者 | Yamaguchi M, Ito A, Okamoto N, Kawabe Y, Kamihira M. |
| タイトル | Heat-inducible transgene expression system incorporating a positive feedback loop of transcriptional amplification for hyperthermia-induced gene therapy. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
One of the major goals of gene therapy is to regulate the expression of therapeutic genes in desired cells or tissues. For this purpose, heat-inducible vectors have been exploited for cancer gene therapy combined with hyperthermia, which can result in considerable improvement of therapeutic effects. In the present study, we constructed a novel heat-inducible gene expression system incorporating a transactivation system with a positive feedback loop of transcriptional amplification. The target gene expression mediated by the transactivator under the control of a heat shock protein 70B' promoter is enhanced by self-promoted transactivator gene expression. This expression system showed tight control of target gene expression together with high-level expression; enhanced expression of the reporter gene was observed in transfected cells upon heat treatment, while negligible gene expression was detected in non-heated cells. When a therapeutic gene was used as the target gene, a considerable cytotoxic effect was observed after heat treatment of cancer cells transfected with the plasmids. The heat-induced transgene expression system is a promising new approach for the development of both a safe and effective vector for hyperthermia-based cancer gene therapy. |
| 巻・号 | 114(4) |
| ページ | 460-5 |
| 公開日 | 2012-10-1 |
| DOI | 10.1016/j.jbiosc.2012.05.006 |
| PII | S1389-1723(12)00202-2 |
| PMID | 22658541 |
| MeSH | Gene Expression* Genes, Reporter Genetic Therapy / methods* Genetic Vectors* HeLa Cells Heat-Shock Proteins / genetics* Hot Temperature Humans Neoplasms / genetics Neoplasms / therapy* Plasmids Promoter Regions, Genetic Trans-Activators / genetics Transfection Transgenes |
| IF | 2.366 |
| 引用数 | 8 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |