| RRC ID |
43882
|
| 著者 |
Yamaguchi M, Ito A, Okamoto N, Kawabe Y, Kamihira M.
|
| タイトル |
Heat-inducible transgene expression system incorporating a positive feedback loop of transcriptional amplification for hyperthermia-induced gene therapy.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
One of the major goals of gene therapy is to regulate the expression of therapeutic genes in desired cells or tissues. For this purpose, heat-inducible vectors have been exploited for cancer gene therapy combined with hyperthermia, which can result in considerable improvement of therapeutic effects. In the present study, we constructed a novel heat-inducible gene expression system incorporating a transactivation system with a positive feedback loop of transcriptional amplification. The target gene expression mediated by the transactivator under the control of a heat shock protein 70B' promoter is enhanced by self-promoted transactivator gene expression. This expression system showed tight control of target gene expression together with high-level expression; enhanced expression of the reporter gene was observed in transfected cells upon heat treatment, while negligible gene expression was detected in non-heated cells. When a therapeutic gene was used as the target gene, a considerable cytotoxic effect was observed after heat treatment of cancer cells transfected with the plasmids. The heat-induced transgene expression system is a promising new approach for the development of both a safe and effective vector for hyperthermia-based cancer gene therapy.
|
| 巻・号 |
114(4)
|
| ページ |
460-5
|
| 公開日 |
2012-10-1
|
| DOI |
10.1016/j.jbiosc.2012.05.006
|
| PII |
S1389-1723(12)00202-2
|
| PMID |
22658541
|
| MeSH |
Gene Expression*
Genes, Reporter
Genetic Therapy / methods*
Genetic Vectors*
HeLa Cells
Heat-Shock Proteins / genetics*
Hot Temperature
Humans
Neoplasms / genetics
Neoplasms / therapy*
Plasmids
Promoter Regions, Genetic
Trans-Activators / genetics
Transfection
Transgenes
|
| IF |
2.366
|
| 引用数 |
8
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa |
