| RRC ID |
43912
|
| 著者 |
Hasebe A, Nakamura Y, Tashima H, Takahashi K, Iijima M, Yoshimoto N, Ting K, Kuroda S, Niimi T.
|
| タイトル |
The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
NELL1 is a secretory osteogenic protein containing several structural motifs that suggest that it functions as an extracellular matrix component. To determine the mechanisms underlying NELL1-induced osteoblast differentiation, we examined the cell-adhesive activity of NELL1 using a series of recombinant NELL1 proteins. We demonstrated that NELL1 promoted osteoblastic cell adhesion through at least three cell-binding domains located in the C-terminal region of NELL1. Adhesion of cells to NELL1 was strongly inhibited by function-blocking antibodies against integrin α3 and β1 subunits, suggesting that osteoblastic cells adhered to NELL1 through integrin α3β1. Further, focal adhesion kinase activation is involved in NELL1 signaling.
|
| 巻・号 |
586(16)
|
| ページ |
2500-6
|
| 公開日 |
2012-7-30
|
| DOI |
10.1016/j.febslet.2012.06.014
|
| PII |
S0014-5793(12)00492-9
|
| PMID |
22728432
|
| MeSH |
3T3 Cells
Animals
Calcium-Binding Proteins / chemistry*
Calcium-Binding Proteins / physiology
Cell Adhesion
Cell Line, Tumor
Dose-Response Relationship, Drug
Gene Expression Regulation*
Glycoproteins / chemistry*
Glycoproteins / physiology
Humans
Integrin alpha3beta1 / biosynthesis*
MAP Kinase Signaling System
Mice
Mice, Inbred C3H
Models, Biological
Osteoblasts / cytology*
Plasmids / metabolism
Protein Structure, Tertiary
Recombinant Proteins / chemistry
Signal Transduction
|
| IF |
3.057
|
| 引用数 |
15
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
