論文 - 詳細
| RRC ID | 43951 |
|---|---|
| 著者 | Iizuka M, Ayaori M, Uto-Kondo H, Yakushiji E, Takiguchi S, Nakaya K, Hisada T, Sasaki M, Komatsu T, Yogo M, Kishimoto Y, Kondo K, Ikewaki K. |
| タイトル | Astaxanthin enhances ATP-binding cassette transporter A1/G1 expressions and cholesterol efflux from macrophages. |
| ジャーナル | J Nutr Sci Vitaminol (Tokyo) |
| Abstract |
ATP-binding cassette transporters (ABC) A1 and G1 are key molecules in cholesterol efflux from macrophages, which is an initial step of reverse cholesterol transport (RCT), a major anti-atherogenic property of high-density lipoprotein (HDL). Astaxanthin is one of the naturally occurring carotenoids responsible for the pink-red pigmentation in a variety of living organisms. Although astaxanthin is known to be a strong antioxidant, it remains unclear through what mechanism of action it affects cholesterol homeostasis in macrophages. We therefore investigated the effects of astaxanthin on cholesterol efflux and ABCA1/G1 expressions in macrophages. Astaxanthin enhanced both apolipoprotein (apo) A-I- and HDL-mediated cholesterol efflux from RAW264.7 cells. In supporting these enhanced cholesterol efflux mechanisms, astaxanthin promoted ABCA1/G1 expression in various macrophages. In contrast, peroxisome proliferator-activated receptor γ, liver X receptor (LXR) α and LXRβ levels remained unchanged by astaxanthin. An experiment using actinomycin D demonstrated that astaxanthin transcriptionally induced ABCA1/G1 expression, and oxysterol depletion caused by overexpression of cholesterol sulfotransferase further revealed that these inductions in ABCA1/G1 were independent of LXR-mediated pathways. Finally, we performed luciferase assays using human ABCA1/G1 promoter-reporter constructs to reveal that astaxanthin activated both promoters irrespective of the presence or absence of LXR-responsive elements, indicating LXR-independence of these activations. In conclusion, astaxanthin increased ABCA1/G1 expression, thereby enhancing apoA-I/HDL-mediated cholesterol efflux from the macrophages in an LXR-independent manner. In addition to the anti-oxidative properties, the potential cardioprotective properties of astaxanthin might therefore be associated with an enhanced anti-atherogenic function of HDL. |
| 巻・号 | 58(2) |
| ページ | 96-104 |
| 公開日 | 2012-1-1 |
| DOI | 10.3177/jnsv.58.96 |
| PII | DN/JST.JSTAGE/jnsv/58.96 |
| PMID | 22790567 |
| MeSH | ATP Binding Cassette Transporter 1 ATP Binding Cassette Transporter, Subfamily G, Member 1 ATP-Binding Cassette Transporters / genetics* ATP-Binding Cassette Transporters / physiology Animals Antioxidants Apolipoprotein A-I / drug effects Apolipoprotein A-I / physiology Cardiotonic Agents Cell Line Cholesterol / metabolism* Gene Expression / drug effects* Lipoproteins / genetics* Lipoproteins / physiology Lipoproteins, HDL / drug effects Lipoproteins, HDL / physiology Liver X Receptors Macrophages / drug effects Macrophages / metabolism* Mice Mice, Inbred C57BL Orphan Nuclear Receptors / physiology Xanthophylls / pharmacology |
| IF | 1.424 |
| 引用数 | 22 |
| WOS 分野 | NUTRITION & DIETETICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) THP-1(RCB1189) |