| RRC ID |
43955
|
| Author |
Hagiwara K, Nakata M, Koyama Y, Sato T.
|
| Title |
The effects of coating pDNA/chitosan complexes with chondroitin sulfate on physicochemical characteristics and cell transfection.
|
| Journal |
Biomaterials
|
| Abstract |
pDNA/chitosan complexes have been investigated as promising non-viral vectors for gene delivery. However, an increase in transfection efficiency and enhancement of physicochemical stability are required for their practical use. In this study, chondroitin sulfate (CS) was employed as a coating agent to increase the stability and transfection efficiency of a pDNA/chitosan complex. The pDNA/chitosan/CS ternary complexes formed with six kinds of CSs having different limiting viscosities (0.2-1.6) and sulfation degrees (5.0-7.0%) showed considerable differences in particle size, surface charge, and morphology. Among them, CS having a medium limiting viscosity (0.5-0.6) and a high sulfation degree (6.9%) showed significant enhancements in cell transfection efficiency. Analyses of cellular uptake and intracellular trafficking revealed that increased cellular uptake via macropinocytosis, together with reduced entry into lysosomes, may explain the promotion of transfection efficiency of ternary complexes.
|
| Volume |
33(29)
|
| Pages |
7251-60
|
| Published |
2012-10-1
|
| DOI |
10.1016/j.biomaterials.2012.06.040
|
| PII |
S0142-9612(12)00690-4
|
| PMID |
22796168
|
| MeSH |
Animals
COS Cells
Cattle
Cell Line
Cell Line, Tumor
Chitosan / chemistry*
Chlorocebus aethiops
Chondroitin Sulfates / chemistry*
DNA / chemistry*
Gene Transfer Techniques*
Humans
Microscopy, Atomic Force / methods
Nanoparticles / chemistry
Pinocytosis
Polysaccharides / chemistry
Sepharose / chemistry
Transfection
Viscosity
|
| IF |
10.317
|
| Times Cited |
33
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
HuH-7(RCB1366) |
