論文 - 詳細
| RRC ID | 43962 |
|---|---|
| 著者 | Ogasawara J, Sakurai T, Kizaki T, Ishibashi Y, Izawa T, Sumitani Y, Ishida H, Radak Z, Haga S, Ohno H. |
| タイトル | Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:In adipose cells, adipose triglyceride lipase (ATGL) catalyzes the first step in adipocyte triacylglyceride hydrolysis, thereby regulating both basal and hormone-stimulated lipolysis. However, little is known about the molecular mechanism(s) underlying habitual exercise-induced adaptive modulation of ATGL in white adipocytes via alteration in transcription regulator and lipolytic cofactors. METHODOLOGY/PRINCIPAL RESULTS:Male Wistar rats were randomly divided into 2 groups a sedentary control group (CG) and a habitual exercise group (EG). The EG was subjected to running on a treadmill set at 5 days per week for 9 weeks. The CG was not subjected to running on a treadmill. In the EG, levels of ATGL mRNA and protein were elevated with a significant increase in lipolysis compared with the CG, accompanied by a significant increase in associations of CGI-58 with ATGL protein. Under these conditions, an upregulation of peroxisome proliferation-activated receptorg-2 (PPARg-2) was observed. In the EG, the addition of rosiglitazone further significantly increased the levels of ATGL protein compared with the CG. However, attenuated levels of the ATGL protein in adipocytes were obtained by the addition of insulin, which is known to inhibit the expression of ATGL, in both types of groups. Actually, levels of plasma insulin were significantly reduced in the EG compared with the CG. CONCLUSIONS:These data suggest that elevated levels of ATGL are involved in the exercise-induced enhancement of lipolysis in primary adipocytes. The exact mechanism(s) underlying this phenomenon is associated, at least in part, with upregulated transcriptional activation of PPARg-2. In addition, exercise-induced lower circulation levels of insulin also correlate with habitual exercise-induced higher levels of ATGL in primary adipocytes. |
| 巻・号 | 7(7) |
| ページ | e40876 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0040876 |
| PII | PONE-D-11-17678 |
| PMID | 22815850 |
| PMC | PMC3397928 |
| MeSH | Acyltransferases Adipocytes / drug effects Adipocytes / enzymology* Animals Body Weight / drug effects Carrier Proteins / metabolism DNA / metabolism Epididymis / cytology* Gene Expression Regulation, Enzymologic / drug effects HeLa Cells Humans Insulin / blood Insulin / pharmacology Lipase / genetics Lipase / metabolism* Lipolysis* / drug effects Male Organ Size / drug effects PPAR gamma / genetics PPAR gamma / metabolism Perilipin-1 Phosphoproteins / metabolism Physical Conditioning, Animal* Protein Binding / drug effects RNA, Messenger / genetics RNA, Messenger / metabolism Rats Rats, Wistar Rosiglitazone Sterol Esterase / metabolism Thiazolidinediones / pharmacology |
| IF | 2.74 |
| 引用数 | 24 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |