論文 - 詳細
| RRC ID | 43978 |
|---|---|
| 著者 | Matsunawa M, Akagi D, Uno S, Endo-Umeda K, Yamada S, Ikeda K, Makishima M. |
| タイトル | Vitamin D receptor activation enhances benzo[a]pyrene metabolism via CYP1A1 expression in macrophages. |
| ジャーナル | Drug Metab Dispos |
| Abstract |
Benzo[a]pyrene (BaP) activates the aryl hydrocarbon (AHR) and induces the expression of genes involved in xenobiotic metabolism, including CYP1A1. CYP1A1 is involved not only in BaP detoxification but also in metabolic activation, which results in DNA adduct formation. Vitamin D receptor (VDR) belongs to the NR1I subfamily of the nuclear receptor superfamily, which also regulates expression of xenobiotic metabolism genes. We investigated the cross-talk between AHR and VDR signaling pathways and found that 1α,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)], a potent physiological VDR agonist, enhanced BaP-induced transcription of CYP1A1 in human monocytic U937 cells and THP-1 cells, breast cancer cells, and kidney epithelium-derived cells. 1,25(OH)(2)D(3) alone did not induce CYP1A1, and 1,25(OH)(2)D(3) plus BaP did not increase CYP1A2 or CYP1B1 mRNA expression in U937 cells. The combination of 1,25(OH)(2)D(3) and BaP increased CYP1A1 protein levels, BaP hydroxylation activity, and BaP-DNA adduct formation in U937 cells and THP-1 cells more effectively than BaP alone. The combined effect of 1,25(OH)(2)D(3) and BaP on CYP1A1 mRNA expression in U937 cells and/or THP-1 cells was inhibited by VDR knockdown, VDR antagonists, and α-naphthoflavone, an AHR antagonist. Electrophoretic mobility shift assays and chromatin immunoprecipitation assays showed that VDR directly bound to an everted repeat (ER) 8 motif in the human CYP1A1 promoter. Thus, CYP1A1 is a novel VDR target gene involved in xenobiotic metabolism. Induction of CYP1A1 by the activation of VDR and AHR may contribute to BaP-mediated toxicity and the physiological function of this enzyme. |
| 巻・号 | 40(11) |
| ページ | 2059-66 |
| 公開日 | 2012-11-1 |
| DOI | 10.1124/dmd.112.046839 |
| PII | dmd.112.046839 |
| PMID | 22837390 |
| MeSH | Benzo(a)pyrene / adverse effects Benzo(a)pyrene / metabolism* Benzo(a)pyrene / pharmacology Calcitriol / genetics Calcitriol / metabolism Cell Line Cell Line, Tumor Cytochrome P-450 CYP1A1 / biosynthesis* Cytochrome P-450 CYP1A1 / genetics* Cytochrome P-450 CYP1A1 / metabolism HEK293 Cells Humans Inactivation, Metabolic / genetics* MCF-7 Cells Macrophages / drug effects Macrophages / enzymology Macrophages / metabolism* Promoter Regions, Genetic / drug effects Promoter Regions, Genetic / genetics Receptors, Aryl Hydrocarbon / genetics Receptors, Aryl Hydrocarbon / metabolism Receptors, Calcitriol / agonists Receptors, Calcitriol / genetics Receptors, Calcitriol / metabolism* Retinoid X Receptors / genetics Retinoid X Receptors / metabolism Transcription, Genetic / drug effects Transcription, Genetic / genetics U937 Cells |
| IF | 3.231 |
| 引用数 | 20 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | U-937 DE-4(RCB0435) THP-1RCB1189) |