論文 - 詳細
| RRC ID | 43980 |
|---|---|
| 著者 | Tsukasaki M, Yamada A, Yoshimura K, Miyazono A, Yamamoto M, Takami M, Miyamoto Y, Morimura N, Kamijo R. |
| タイトル | Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Nephronectin (Npnt) is an extracellular matrix protein known to be a ligand for the integrin α8β1. We previously demonstrated that Npnt expression was suppressed by TGF-β through ERK1/2 and JNK in osteoblasts. In this study, we found that inhibition of a TGF-β type I receptor (TGF-β R1, Alk5) by a specific inhibitor {2-[3-(6-Methylpyridin-2-yl)-1H-pyrazol-4-yl]-1,5-naphthyridine} strongly induced Npnt expression in osteoblast-like MC3T3-E1 cells. The Alk5 inhibitor-induced increase of Npnt expression occurred in both time- and dose-dependent manners, while that expression was also induced by introduction of an siRNA for Smad2, a central intracellular mediator of TGF-β signaling. These results suggest that the expression of Npnt is regulated by the Alk5-SMAD signaling pathway in osteoblasts. |
| 巻・号 | 425(2) |
| ページ | 390-2 |
| 公開日 | 2012-8-24 |
| DOI | 10.1016/j.bbrc.2012.07.106 |
| PII | S0006-291X(12)01413-1 |
| PMID | 22842459 |
| MeSH | 3T3 Cells Animals Extracellular Matrix Proteins / genetics* Gene Expression Regulation Mice Osteoblasts / metabolism* Protein Serine-Threonine Kinases / metabolism RNA, Small Interfering / genetics Receptor, Transforming Growth Factor-beta Type I Receptors, Transforming Growth Factor beta / metabolism Signal Transduction Smad2 Protein / genetics Smad2 Protein / metabolism* Transforming Growth Factor beta / pharmacology Transforming Growth Factor beta / physiology |
| IF | 2.985 |
| 引用数 | 6 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |