| RRC ID |
43980
|
| 著者 |
Tsukasaki M, Yamada A, Yoshimura K, Miyazono A, Yamamoto M, Takami M, Miyamoto Y, Morimura N, Kamijo R.
|
| タイトル |
Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Nephronectin (Npnt) is an extracellular matrix protein known to be a ligand for the integrin α8β1. We previously demonstrated that Npnt expression was suppressed by TGF-β through ERK1/2 and JNK in osteoblasts. In this study, we found that inhibition of a TGF-β type I receptor (TGF-β R1, Alk5) by a specific inhibitor {2-[3-(6-Methylpyridin-2-yl)-1H-pyrazol-4-yl]-1,5-naphthyridine} strongly induced Npnt expression in osteoblast-like MC3T3-E1 cells. The Alk5 inhibitor-induced increase of Npnt expression occurred in both time- and dose-dependent manners, while that expression was also induced by introduction of an siRNA for Smad2, a central intracellular mediator of TGF-β signaling. These results suggest that the expression of Npnt is regulated by the Alk5-SMAD signaling pathway in osteoblasts.
|
| 巻・号 |
425(2)
|
| ページ |
390-2
|
| 公開日 |
2012-8-24
|
| DOI |
10.1016/j.bbrc.2012.07.106
|
| PII |
S0006-291X(12)01413-1
|
| PMID |
22842459
|
| MeSH |
3T3 Cells
Animals
Extracellular Matrix Proteins / genetics*
Gene Expression Regulation
Mice
Osteoblasts / metabolism*
Protein Serine-Threonine Kinases / metabolism
RNA, Small Interfering / genetics
Receptor, Transforming Growth Factor-beta Type I
Receptors, Transforming Growth Factor beta / metabolism
Signal Transduction
Smad2 Protein / genetics
Smad2 Protein / metabolism*
Transforming Growth Factor beta / pharmacology
Transforming Growth Factor beta / physiology
|
| IF |
2.985
|
| 引用数 |
6
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
