| RRC ID |
43999
|
| 著者 |
Shiwaku Y, Anada T, Yamazaki H, Honda Y, Morimoto S, Sasaki K, Suzuki O.
|
| タイトル |
Structural, morphological and surface characteristics of two types of octacalcium phosphate-derived fluoride-containing apatitic calcium phosphates.
|
| ジャーナル |
Acta Biomater
|
| Abstract |
Octacalcium phosphate (OCP) has been reported to stimulate bone regeneration during hydrolysis into hydroxyapatite (HA). The present study was designed to characterize structural, morphological and surface properties of fluoride-containing apatitic calcium phosphates (CaP) obtained through OCP hydrolysis or direct precipitation of OCP in the presence of 12-230ppm of fluoride (F). The products were characterized by chemical analysis, X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and Fourier transform infrared spectroscopy (FTIR) as well as measurements of surface area, solubility, osteoblastic activities and bovine serum albumin (BSA) adsorption. XRD analysis re-confirmed that both preparations yielded more apatitic CaP with a higher concentration of F. However, the co-precipitated products (CF-CaP) maintained the properties of OCP, in particular the solubility, whereas the hydrolysis products (HF-CaP) had the characteristics of fluoridated apatite. The crystals of plate-like OCP were changed to the crystals of rod-like CF-CaP and small irregular HF-CaP with the advance of the hydrolysis. The SAED analysis detected both OCP and apatite crystals even in the most hydrolyzed CF-CaP. Mouse bone marrow stromal ST-2 cells grew better on CF-CaP compared with HF-CaP. BSA adsorption was inhibited on HF-CaP more than on CF-CaP. These results show that OCP produces physicochemically distinct apatitic fluoridated CaP during hydrolysis, regarding the structure, the crystal morphology and the protein adsorption, depending on the fluoride introduction route, which provides biologically interesting material.
|
| 巻・号 |
8(12)
|
| ページ |
4417-25
|
| 公開日 |
2012-12-1
|
| DOI |
10.1016/j.actbio.2012.07.041
|
| PII |
S1742-7061(12)00360-1
|
| PMID |
22868193
|
| MeSH |
Animals
Apatites* / chemistry
Apatites* / pharmacology
Calcium Phosphates* / chemistry
Calcium Phosphates* / pharmacology
Cattle
Cell Line
Fluorides* / chemistry
Fluorides* / pharmacology
Mice
Osteoblasts / cytology
Osteoblasts / metabolism*
Serum Albumin, Bovine / chemistry
Serum Albumin, Bovine / pharmacology
Surface Properties
X-Ray Diffraction
|
| IF |
7.242
|
| 引用数 |
26
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
