| RRC ID |
44030
|
| Author |
Yang XY, Huang CC, Kan QM, Li Y, Liu D, Zhang XC, Sato T, Yamagata S, Yamagata T.
|
| Title |
Calcium regulates caveolin-1 expression at the transcriptional level.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca(2+)/calcineurin/NFAT.
|
| Volume |
426(3)
|
| Pages |
334-41
|
| Published |
2012-9-28
|
| DOI |
10.1016/j.bbrc.2012.08.079
|
| PII |
S0006-291X(12)01598-7
|
| PMID |
22940132
|
| MeSH |
Animals
Calcineurin / metabolism
Calcium / metabolism*
Calcium / pharmacology
Calcium Channels, L-Type / metabolism
Calcium Signaling*
Caveolin 1 / genetics*
Cell Line, Tumor
Mice
NFATC Transcription Factors
Transcription, Genetic*
|
| IF |
2.985
|
| Times Cited |
8
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
NIH3T3 |
