論文 - 詳細
| RRC ID | 44040 |
|---|---|
| 著者 | Watari A, Yagi K, Kondoh M. |
| タイトル | A simple reporter assay for screening claudin-4 modulators. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Claudin-4, a member of a tetra-transmembrane protein family that comprises 27 members, is a key functional and structural component of the tight junction-seal in mucosal epithelium. Modulation of the claudin-4-barrier for drug absorption is now of research interest. Disruption of the claudin-4-seal occurs during inflammation. Therefore, claudin-4 modulators (repressors and inducers) are promising candidates for drug development. However, claudin-4 modulators have never been fully developed. Here, we attempted to design a screening system for claudin-4 modulators by using a reporter assay. We prepared a plasmid vector coding a claudin-4 promoter-driven luciferase gene and established stable reporter gene-expressing cells. We identified thiabendazole, carotene and curcumin as claudin-4 inducers, and potassium carbonate as a claudin-4 repressor by using the reporter cells. They also increased or decreased, respectively, the integrity of the tight junction-seal in Caco-2 cells. This simple reporter system will be a powerful tool for the development of claudin-4 modulators. |
| 巻・号 | 426(4) |
| ページ | 454-60 |
| 公開日 | 2012-10-5 |
| DOI | 10.1016/j.bbrc.2012.08.083 |
| PII | S0006-291X(12)01602-6 |
| PMID | 22960168 |
| MeSH | Cell Line, Tumor Claudin-4 / agonists* Claudin-4 / antagonists & inhibitors* Drug Evaluation, Preclinical / methods Gene Expression / drug effects Genes, Reporter / drug effects* Humans Luciferases / genetics Plasmids / genetics Promoter Regions, Genetic / drug effects |
| IF | 2.985 |
| 引用数 | 8 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MCF7(RCB1904) |