論文 - 詳細
| RRC ID | 44041 |
|---|---|
| 著者 | Takano M, Horiuchi T, Nagai J, Yumoto R. |
| タイトル | Effect of cigarette smoke extract on insulin transport in alveolar epithelial cell line A549. |
| ジャーナル | Lung |
| Abstract |
BACKGROUND:The main purpose of this study was to evaluate the effect of cigarette smoke extract (CSE) on insulin transport in alveolar epithelial cells. METHODS:We first examined the effect of CSE pretreatment on cell viability, mRNA expression, and lamellar body structures in A549 cells. Then the effect of CSE pretreatment on FITC-insulin transport was examined. RESULTS:When A549 cells were treated with 30 μg/ml of CSE for 48 h, the expression of some mRNAs abundantly expressed in type II alveolar epithelial cells such as surfactant protein B was significantly increased. Lamellar bodylike structures became more evident with CSE treatment. FITC-insulin uptake from the apical side and subsequent efflux to the basal side was enhanced by CSE treatment in A549 cells. The enhancing effect of CSE on FITC-insulin uptake was concentration-dependent and reversible. A concentration-dependent enhancing effect of CSE on FITC-insulin uptake was also observed in normal, primary cultured alveolar type II epithelial cells isolated from rats. CONCLUSIONS:Treatment of A549 cells by CSE may direct the cells to a more type II-like phenotype. In accordance with this observation, FITC-insulin uptake was enhanced by CSE treatment. These results may partly explain the higher insulin absorption from the lung in smokers than in nonsmokers. |
| 巻・号 | 190(6) |
| ページ | 651-9 |
| 公開日 | 2012-12-1 |
| DOI | 10.1007/s00408-012-9413-9 |
| PMID | 22960792 |
| MeSH | Animals Biological Transport / drug effects Cell Line Cell Survival Cells, Cultured Dose-Response Relationship, Drug Epithelial Cells / metabolism Humans Insulin / metabolism* Microscopy, Confocal Pulmonary Alveoli / metabolism* Pulmonary Alveoli / ultrastructure RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Smoke* / analysis Tobacco / chemistry* |
| IF | 1.817 |
| 引用数 | 5 |
| WOS 分野 | RESPIRATORY SYSTEM |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |