RRC ID 44057
Author Sugaya S, Kakegawa S, Fukushima S, Yamada M, Seki M.
Title Micropatterning of hydrogels on locally hydrophilized regions on PDMS by stepwise solution dipping and in situ gelation.
Journal Langmuir
Abstract This study presents a simple but highly versatile method of fabricating picoliter-volume hydrogel patterns on poly(dimethylsiloxane) (PDMS) substrates. Hydrophilic regions were prepared on hydrophobic PDMS plates by trapping and melting functional polymer particles and performing subsequent reactions with partially oxidized dextran. Small aliquots of a gelation solution were selectively trapped on the hydrophilic areas by a simple dipping process that was utilized to make thin hydrogel patterns by the in situ gelation of a sol solution. Using this process, we successfully formed calcium alginate, collagen I, and chitosan hydrogels with a thickness of several micrometers and shapes that followed the hydrophilized regions. In addition, alginate and collagen gel patterns were used to capture cells with different adhesion properties selectively on or off the hydrogel structures. The presented strategy could be applicable to the preparation of a variety of hydrogels for the development of functional biosensors, bioreactors, and cell cultivation platforms.
Volume 28(39)
Pages 14073-80
Published 2012-10-2
DOI 10.1021/la3014706
PMID 22991929
MeSH Alginates / chemistry Chitosan / chemistry Collagen Type I / chemistry Dimethylpolysiloxanes / chemistry* Glucuronic Acid / chemistry Hexuronic Acids / chemistry Hydrogels / chemical synthesis Hydrogels / chemistry* Hydrophobic and Hydrophilic Interactions Solutions
IF 3.557
Times Cited 11
WOS Category MATERIALS SCIENCE, MULTIDISCIPLINARY CHEMISTRY, MULTIDISCIPLINARY CHEMISTRY, PHYSICAL
Resource
Human and Animal Cells PC-12(RCB0009) NIH3T3/14-1(RCB0056)