| RRC ID |
44076
|
| 著者 |
Miyazawa K, He F, Yoda K, Hiramatsu M.
|
| タイトル |
Potent effects of, and mechanisms for, modification of crosstalk between macrophages and adipocytes by lactobacilli.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
The murine macrophage-like cell line J774.1 was treated with heat-killed cells of Lactobacillus GG (LGG) and L. gasseri TMC0356 (TMC 0356). Interleukin (IL)-6, IL-12, and tumor necrosis factor-α were profiled from the J774.1 cells using enzyme-linked immunosorbent assay methods. The conditioned medium from cultured J774.1 cells was transferred to the preadipocyte cell line 3T3-L1 (which is a mouse embryonic fibroblast-adipose-like cell line). Growth and differentiation of 3T3-L1 cells were monitored by analyzing lipid accumulation and expression of peroxisome proliferator-activated receptor (PPAR)-γ mRNA. The medium conditioned by 3T3-L1 cells was added to J774.1 cells and the cytokines in the supernatant analyzed. Compared with that of cells exposed to a PBS-conditioned medium, lipid accumulation in 3T3-L1 cells was significantly suppressed in a dose-dependent manner by each medium that had been conditioned with LGG and TMC0356. PPAR-γ mRNA expression in 3T3-L1 cells was also significantly downregulated (P < 0.01, P < 0.05, respectively). The conditioned medium of 3T3-L1 adipose phenotype significantly stimulated production of IL-6 and IL-12 in J774.1 cells treated with LGG and TMC0356. These results suggest that lactobacilli may suppress differentiation of preadipocytes through macrophage activation and alter the immune responses of macrophages to adipose cells.
|
| 巻・号 |
56(12)
|
| ページ |
847-54
|
| 公開日 |
2012-12-1
|
| DOI |
10.1111/j.1348-0421.2012.00512.x
|
| PMID |
23017059
|
| MeSH |
Adipocytes / chemistry
Adipocytes / cytology*
Adipocytes / physiology
Animals
Cell Differentiation*
Cell Line
Cell Proliferation*
Culture Media, Conditioned / chemistry
Cytokines / analysis
Gene Expression Profiling
Lactobacillus / immunology*
Lipids / analysis
Macrophages / cytology
Macrophages / immunology*
Macrophages / microbiology*
Mice
PPAR gamma / biosynthesis
|
| IF |
1.566
|
| 引用数 |
6
|
|
WOS 分野
|
MICROBIOLOGY
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
J774.1 |
