論文 - 詳細
| RRC ID | 44076 |
|---|---|
| 著者 | Miyazawa K, He F, Yoda K, Hiramatsu M. |
| タイトル | Potent effects of, and mechanisms for, modification of crosstalk between macrophages and adipocytes by lactobacilli. |
| ジャーナル | Microbiol Immunol |
| Abstract |
The murine macrophage-like cell line J774.1 was treated with heat-killed cells of Lactobacillus GG (LGG) and L. gasseri TMC0356 (TMC 0356). Interleukin (IL)-6, IL-12, and tumor necrosis factor-α were profiled from the J774.1 cells using enzyme-linked immunosorbent assay methods. The conditioned medium from cultured J774.1 cells was transferred to the preadipocyte cell line 3T3-L1 (which is a mouse embryonic fibroblast-adipose-like cell line). Growth and differentiation of 3T3-L1 cells were monitored by analyzing lipid accumulation and expression of peroxisome proliferator-activated receptor (PPAR)-γ mRNA. The medium conditioned by 3T3-L1 cells was added to J774.1 cells and the cytokines in the supernatant analyzed. Compared with that of cells exposed to a PBS-conditioned medium, lipid accumulation in 3T3-L1 cells was significantly suppressed in a dose-dependent manner by each medium that had been conditioned with LGG and TMC0356. PPAR-γ mRNA expression in 3T3-L1 cells was also significantly downregulated (P < 0.01, P < 0.05, respectively). The conditioned medium of 3T3-L1 adipose phenotype significantly stimulated production of IL-6 and IL-12 in J774.1 cells treated with LGG and TMC0356. These results suggest that lactobacilli may suppress differentiation of preadipocytes through macrophage activation and alter the immune responses of macrophages to adipose cells. |
| 巻・号 | 56(12) |
| ページ | 847-54 |
| 公開日 | 2012-12-1 |
| DOI | 10.1111/j.1348-0421.2012.00512.x |
| PMID | 23017059 |
| MeSH | Adipocytes / chemistry Adipocytes / cytology* Adipocytes / physiology Animals Cell Differentiation* Cell Line Cell Proliferation* Culture Media, Conditioned / chemistry Cytokines / analysis Gene Expression Profiling Lactobacillus / immunology* Lipids / analysis Macrophages / cytology Macrophages / immunology* Macrophages / microbiology* Mice PPAR gamma / biosynthesis |
| IF | 1.566 |
| 引用数 | 6 |
| WOS 分野 | MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | J774.1 |