Reference - Detail
| RRC ID | 44089 |
|---|---|
| Author | Ozasa R, Ohno J, Iwahashi T, Taniguchi K. |
| Title | Tumor-induced lymphangiogenesis in cervical lymph nodes in oral melanoma-bearing mice. |
| Journal | J Exp Clin Cancer Res |
| Abstract |
BACKGROUND:Metastasis via the lymphatic system is promoted by lymphangiogenesis. Alterations of the lymphatic channels during the progression of metastasis to regional lymph nodes (LNs) remain unexplored. To examine whether tumor-induced LN lymphangiogenesis controls metastasis to regional LNs, we investigated cervical LN metastasis in a mouse model of oral melanoma. METHODS:Injection of B16F10 melanoma cells into mouse tongues replicated spontaneous cervical LN metastasis. We performed histological, immunofluorescent, and histomorphometric analyses of tumor-reactive lymphadenopathy and lymphangiogenesis in tumor-associated LNs. We investigated the expression of vascular endothelial growth factor (VEGF)-C and its receptor, VEGF receptor-3 (VEGFR-3), in tumor cells and tissues, and LNs by reverse transcription polymerase chain reaction and immunofluorescence. RESULTS:Tumor-associated LNs comprised sentinel LNs (SLNs) before and after tumor cell invasion (tumor-bearing SLNs), and LNs adjacent or contralateral to tumor-bearing SLNs. Extensive lymphangiogenesis appeared in SLNs before evidence of metastasis. After metastasis was established in SLNs, both LNs adjacent and contralateral to tumor-bearing SLNs demonstrated lymphangiogenesis. Interaction between VEGF-C-positive melanoma cells and VEGFR-3-positive lymphatic vessels was evident in tumor-associated LNs. CONCLUSIONS:LN lymphangiogenesis contributes a progression of tumor metastasis from SLNs to other regional LNs. |
| Volume | 31(1) |
| Pages | 83 |
| Published | 2012-10-2 |
| DOI | 10.1186/1756-9966-31-83 |
| PII | 1756-9966-31-83 |
| PMID | 23031500 |
| PMC | PMC3523084 |
| MeSH | Animals Female Gene Expression Regulation, Neoplastic Humans Lymph Nodes / metabolism Lymph Nodes / pathology Lymphangiogenesis* Lymphatic Metastasis / pathology* Melanoma* / metabolism Melanoma* / pathology Melanoma, Experimental Mice Prognosis Uterine Cervical Neoplasms* / metabolism Uterine Cervical Neoplasms* / pathology Uterine Cervical Neoplasms* / secondary Vascular Endothelial Growth Factor C / metabolism Vascular Endothelial Growth Factor Receptor-3 / metabolism |
| IF | 7.068 |
| Times Cited | 11 |
| WOS Category | ONCOLOGY |
| Resource | |
| Human and Animal Cells | B16/F10(RCB2630) |