RRC ID 4409
Author Shteingauz A, Cohen E, Biala Y, Treinin M.
Title The BTB-MATH protein BATH-42 interacts with RIC-3 to regulate maturation of nicotinic acetylcholine receptors.
Journal J. Cell. Sci.
Abstract RIC-3 is a member of a conserved family of proteins that affect nicotinic acetylcholine receptor maturation. In yeast and in vitro, BATH-42, a BTB- and MATH-domain-containing protein, interacts with RIC-3. BATH-42 is also known to interact with the CUL-3 ubiquitin ligase complex. Loss of BATH-42 function leads to increased RIC-3 expression and decreased activity of nicotinic acetylcholine receptors in Caenorhabditis elegans vulva muscles. Increased expression of RIC-3 is deleterious for activity and distribution of nicotinic acetylcholine receptors, and thus the effects of BATH-42 loss of function on RIC-3 expression explain the associated reduction in receptor activity. Overexpression of BATH-42 is also detrimental to nicotinic acetylcholine receptor function, leading to decreased pharyngeal pumping. This effect depends on the C-terminus of RIC-3 and on CUL-3. Thus, our work suggests that BATH-42 targets RIC-3 to degradation via CUL-3-mediated ubiquitylation. This demonstrates the importance of regulation of RIC-3 levels, and identifies a mechanism that protects cells from the deleterious effects of excess RIC-3.
Volume 122(Pt 6)
Pages 807-12
Published 2009-3-15
DOI 10.1242/jcs.036343
PII jcs.036343
PMID 19223395
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / drug effects Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry* Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / chemistry* Carrier Proteins / metabolism* Female Levamisole / pharmacology Muscles / cytology Muscles / drug effects Muscles / metabolism Mutation / genetics Pharynx / cytology Pharynx / metabolism Protein Binding Protein Structure, Tertiary Protein Transport / drug effects Receptors, Nicotinic / metabolism* Vulva / cytology Vulva / drug effects Vulva / metabolism
IF 4.517
Times Cited 12
C.elegans tm2360