| RRC ID |
44094
|
| Author |
Yokomakura A, Hong J, Ohuchi K, Oh SE, Lee JY, Mano N, Takahashi T, Hwang GW, Naganuma A.
|
| Title |
Increased production of reactive oxygen species by the vacuolar-type (H(+))-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells.
|
| Journal |
J Toxicol Sci
|
| Abstract |
Treatment of the mouse leukemic cell line RAW 264 with bafilomycin A1 or concanamycin A, inhibitors of vacuolar-type (H(+))-ATPases (V-ATPases), significantly increased the production of reactive oxygen species (ROS) and decreased cell viability. These effects were significantly suppressed by the presence of N-acetyl cysteine (NAC), an ROS scavenger. si-RNA mediated knockdown of the gene for the c subunit of the V0 domain of V-ATPase also resulted in an increase in ROS production and a decrease in cell viability. These results suggest that decreased cellular V-ATPase activity decreases cell viability by increasing ROS production in RAW 264 cells.
|
| Volume |
37(5)
|
| Pages |
1045-8
|
| Published |
2012-1-1
|
| DOI |
10.2131/jts.37.1045
|
| PII |
DN/JST.JSTAGE/jts/37.1045
|
| PMID |
23038011
|
| MeSH |
Animals
Cell Line
Cell Survival / drug effects
Enzyme Inhibitors / toxicity*
Macrolides / toxicity*
Mice
Reactive Oxygen Species / metabolism*
Vacuolar Proton-Translocating ATPases / antagonists & inhibitors*
|
| IF |
1.737
|
| Times Cited |
5
|
|
WOS Category
|
TOXICOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
