RRC ID 44103
著者 Ogawa M, Kariya Y, Kitakaze T, Yamaji R, Harada N, Sakamoto T, Hosotani K, Nakano Y, Inui H.
タイトル The preventive effect of β-carotene on denervation-induced soleus muscle atrophy in mice.
ジャーナル Br J Nutr
Abstract Muscle atrophy increases the production of reactive oxygen species and the expression of atrophy-related genes, which are involved in the ubiquitin-proteasome system. In the present study, we investigated the effects of β-carotene on oxidative stress (100 μM-H2O2)-induced muscle atrophy in murine C2C12 myotubes. β-Carotene (10 μM) restored the H2O2-induced decreased levels of myosin heavy chain and tropomyosin (P< 0·05, n 3) and decreased the H2O2-induced increased levels of ubiquitin conjugates. β-Carotene reduced the H2O2-induced increased expression levels of E3 ubiquitin ligases (Atrogin-1 and MuRF1) and deubiquitinating enzymes (USP14 and USP19) (P< 0·05, n 3) and attenuated the H2O2-induced nuclear localisation of FOXO3a. Furthermore, we determined the effects of β-carotene on denervation-induced muscle atrophy. Male ddY mice (8 weeks old, n 30) were divided into two groups and orally pre-administered micelle with or without β-carotene (0·5 mg once daily) for 2 weeks, followed by denervation in the right hindlimb. β-Carotene was further administered once daily until the end of the experiment. At day 3 after denervation, the ratio of soleus muscle mass in the denervated leg to that in the sham leg was significantly higher in β-carotene-administered mice than in control vehicle-administered ones (P< 0·05, n 5). In the denervated soleus muscle, β-carotene administration significantly decreased the expression levels of Atrogin-1, MuRF1, USP14 and USP19 (P< 0·05, n 5) and the levels of ubiquitin conjugates. These results indicate that β-carotene attenuates soleus muscle loss, perhaps by repressing the expressions of Atrogin-1, MuRF1, USP14 and USP19, at the early stage of soleus muscle atrophy.
巻・号 109(8)
ページ 1349-58
公開日 2013-4-28
DOI 10.1017/S0007114512003297
PII S0007114512003297
PMID 23046823
MeSH Analysis of Variance Animals Cells, Cultured Forkhead Box Protein O3 Forkhead Transcription Factors / genetics Forkhead Transcription Factors / metabolism* Gene Expression Regulation Hydrogen Peroxide Male Mice Muscle Denervation Muscle Fibers, Skeletal / drug effects Muscle Fibers, Skeletal / enzymology Muscle Proteins / genetics Muscle Proteins / metabolism* Muscle, Skeletal / innervation* Muscle, Skeletal / metabolism Muscular Atrophy / chemically induced Muscular Atrophy / metabolism Muscular Atrophy / prevention & control* Myoblasts, Skeletal / drug effects Myoblasts, Skeletal / enzymology Oxidative Stress / physiology Reverse Transcriptase Polymerase Chain Reaction Thiobarbituric Acid Reactive Substances / metabolism Ubiquitin Thiolesterase / genetics* Ubiquitin Thiolesterase / metabolism Ubiquitin-Protein Ligase Complexes / genetics Ubiquitin-Protein Ligase Complexes / metabolism* beta Carotene / pharmacology*
IF 3.334
引用数 28
WOS 分野 NUTRITION & DIETETICS
リソース情報
ヒト・動物細胞 C2C12(RCB0987)