| RRC ID |
44123
|
| Author |
Lee J, Miyanaga Y, Ueda M, Hohng S.
|
| Title |
Video-rate confocal microscopy for single-molecule imaging in live cells and superresolution fluorescence imaging.
|
| Journal |
Biophys J
|
| Abstract |
There is no confocal microscope optimized for single-molecule imaging in live cells and superresolution fluorescence imaging. By combining the swiftness of the line-scanning method and the high sensitivity of wide-field detection, we have developed a, to our knowledge, novel confocal fluorescence microscope with a good optical-sectioning capability (1.0 μm), fast frame rates (<33 fps), and superior fluorescence detection efficiency. Full compatibility of the microscope with conventional cell-imaging techniques allowed us to do single-molecule imaging with a great ease at arbitrary depths of live cells. With the new microscope, we monitored diffusion motion of fluorescently labeled cAMP receptors of Dictyostelium discoideum at both the basal and apical surfaces and obtained superresolution fluorescence images of microtubules of COS-7 cells at depths in the range 0-85 μm from the surface of a coverglass.
|
| Volume |
103(8)
|
| Pages |
1691-7
|
| Published |
2012-10-17
|
| DOI |
10.1016/j.bpj.2012.09.014
|
| PII |
S0006-3495(12)01027-2
|
| PMID |
23083712
|
| PMC |
PMC3475383
|
| MeSH |
Animals
COS Cells
Chlorocebus aethiops
Dictyostelium
Fluorescence Resonance Energy Transfer
Microscopy, Confocal / instrumentation
Microscopy, Confocal / methods*
Microscopy, Fluorescence / instrumentation
Microscopy, Fluorescence / methods*
Microtubules / chemistry
Optical Imaging / instrumentation
Optical Imaging / methods*
Receptors, Cyclic AMP / analysis
|
| IF |
3.854
|
| Times Cited |
20
|
|
WOS Category
|
BIOPHYSICS
|
| Resource |
| Human and Animal Cells |
|
