論文 - 詳細
| RRC ID | 44126 |
|---|---|
| 著者 | Kimura T, Yoshida K, Yamamoto C, Suzuki M, Uno T, Isobe M, Naka H, Yasuike S, Satoh M, Kaji T, Uchiyama M. |
| タイトル | Bis(L-cysteinato)zincate(lI) as a coordination compound that induces metallothionein gene transcription without inducing cell-stress-related gene transcription. |
| ジャーナル | J Inorg Biochem |
| Abstract |
Zinc is an essential micronutrient, deficiency of which results in growth retardation, immunodeficiency, and neurological diseases such as dysgeusia. Several zinc coordination compounds are used for zinc supplementation; however, supplemented zinc ions have no specificity and interact with various groups of molecules. Here, we found that, from a library of 30 zinc coordination compounds, bis(L-cysteinato)zincate(II), designated Z01, functioned as a metallothionein (MT) inducer. Z01 induced MT expression mediated by the transcription factor MTF-1, without inducing cell-stress-related heme oxygenase-1 gene expression at specific concentration. The zinc ion was necessary for the MT induction. (65)Zn incorporation following treatment with (65)Zn-labeled Z01 suggested that Z01 did not act as zinc ionophore despite its hydrophilicity. Electrophoretic mobility shift assays revealed that Z01 facilitates MTF-1-MRE complex formation, and, by inference, transfer of zinc from Z01 to MTF-1. Phosphorylated ERK levels were increased by ZnSO(4) treatment but not by Z01. Although our data do not definitely prove that Z01 is an MTF-1-specific activator, our observations suggest that zinc coordination compounds can regulate zinc distribution and act as zinc donors for specific molecules. |
| 巻・号 | 117 |
| ページ | 140-6 |
| 公開日 | 2012-12-1 |
| DOI | 10.1016/j.jinorgbio.2012.07.021 |
| PII | S0162-0134(12)00260-7 |
| PMID | 23085594 |
| MeSH | Animals Cell Line, Tumor Coordination Complexes / chemistry* Coordination Complexes / pharmacology Cysteine / analogs & derivatives* Cysteine / chemistry Cysteine / pharmacology Electrophoretic Mobility Shift Assay Gene Expression* Humans Hydrophobic and Hydrophilic Interactions Metallothionein / genetics* Mice Promoter Regions, Genetic Transcription, Genetic* Transfection Zinc / chemistry* |
| IF | 3.212 |
| 引用数 | 4 |
| WOS 分野 | CHEMISTRY, INORGANIC & NUCLEAR BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hepa 1-6(RCB1638) 293(RCB1637) Hep G2(RCB1648) |