RRC ID |
44126
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著者 |
Kimura T, Yoshida K, Yamamoto C, Suzuki M, Uno T, Isobe M, Naka H, Yasuike S, Satoh M, Kaji T, Uchiyama M.
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タイトル |
Bis(L-cysteinato)zincate(lI) as a coordination compound that induces metallothionein gene transcription without inducing cell-stress-related gene transcription.
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ジャーナル |
J Inorg Biochem
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Abstract |
Zinc is an essential micronutrient, deficiency of which results in growth retardation, immunodeficiency, and neurological diseases such as dysgeusia. Several zinc coordination compounds are used for zinc supplementation; however, supplemented zinc ions have no specificity and interact with various groups of molecules. Here, we found that, from a library of 30 zinc coordination compounds, bis(L-cysteinato)zincate(II), designated Z01, functioned as a metallothionein (MT) inducer. Z01 induced MT expression mediated by the transcription factor MTF-1, without inducing cell-stress-related heme oxygenase-1 gene expression at specific concentration. The zinc ion was necessary for the MT induction. (65)Zn incorporation following treatment with (65)Zn-labeled Z01 suggested that Z01 did not act as zinc ionophore despite its hydrophilicity. Electrophoretic mobility shift assays revealed that Z01 facilitates MTF-1-MRE complex formation, and, by inference, transfer of zinc from Z01 to MTF-1. Phosphorylated ERK levels were increased by ZnSO(4) treatment but not by Z01. Although our data do not definitely prove that Z01 is an MTF-1-specific activator, our observations suggest that zinc coordination compounds can regulate zinc distribution and act as zinc donors for specific molecules.
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巻・号 |
117
|
ページ |
140-6
|
公開日 |
2012-12-1
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DOI |
10.1016/j.jinorgbio.2012.07.021
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PII |
S0162-0134(12)00260-7
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PMID |
23085594
|
MeSH |
Animals
Cell Line, Tumor
Coordination Complexes / chemistry*
Coordination Complexes / pharmacology
Cysteine / analogs & derivatives*
Cysteine / chemistry
Cysteine / pharmacology
Electrophoretic Mobility Shift Assay
Gene Expression*
Humans
Hydrophobic and Hydrophilic Interactions
Metallothionein / genetics*
Mice
Promoter Regions, Genetic
Transcription, Genetic*
Transfection
Zinc / chemistry*
|
IF |
3.212
|
引用数 |
4
|
WOS 分野
|
CHEMISTRY, INORGANIC & NUCLEAR
BIOCHEMISTRY & MOLECULAR BIOLOGY
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リソース情報 |
ヒト・動物細胞 |
|