論文 - 詳細
| RRC ID | 44132 |
|---|---|
| 著者 | Kusano-Arai O, Iwanari H, Mochizuki Y, Nakata H, Kodama T, Kitoh T, Hamakubo T. |
| タイトル | Evaluation of the asparagine synthetase level in leukemia cells by monoclonal antibodies. |
| ジャーナル | Hybridoma (Larchmt) |
| Abstract |
L-Asparaginase (ASNase) is important for the treatment of childhood acute lymphoblastic leukemia. ASNase sensitivity has been shown to correlate with the asparagine synthetase (ASNS) protein content in acute lymphoblastic leukemia cell lines. However, there have been few studies to determine ASNS protein levels in human leukemias, since no appropriate monoclonal antibody is available for such quantitative analysis. In this study, we report the generation of anti-ASNS monoclonal antibodies, which are applicable to flow cytometry and enzyme-linked immunosorbent assay. These monoclonal antibodies should provide a valuable tool for the quantification of ASNS protein level and estimation of ASNase-resistance in leukemia cells. |
| 巻・号 | 31(5) |
| ページ | 325-32 |
| 公開日 | 2012-10-1 |
| DOI | 10.1089/hyb.2012.0014 |
| PMID | 23098298 |
| MeSH | Animals Antibodies, Monoclonal / chemistry Antibodies, Monoclonal / immunology* Antibodies, Monoclonal / isolation & purification Aspartate-Ammonia Ligase / genetics Aspartate-Ammonia Ligase / immunology Aspartate-Ammonia Ligase / metabolism* Baculoviridae / genetics Blotting, Western Cell Line, Tumor Chlorocebus aethiops Enzyme-Linked Immunosorbent Assay Flow Cytometry Genetic Vectors Humans Immunization Mice Precursor Cell Lymphoblastic Leukemia-Lymphoma / enzymology* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / immunology Recombinant Fusion Proteins / metabolism Transfection |
| 引用数 | 2 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMICAL RESEARCH METHODS IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562 MOLT-4(RCB0206) |