RRC ID 44143
著者 Lambrechts D, Roeffaers M, Kerckhofs G, Roberts SJ, Hofkens J, Van de Putte T, Van Oosterwyck H, Schrooten J.
タイトル Fluorescent oxygen sensitive microbead incorporation for measuring oxygen tension in cell aggregates.
ジャーナル Biomaterials
Abstract Molecular oxygen is a main regulator of various cell functions. Imaging methods designed as screening tools for fast, in situ, 3D and non-interfering measurement of oxygen tension in the cellular microenvironment would serve great purpose in identifying and monitoring this vital and pivotal signalling molecule. We describe the use of dual luminophore oxygen sensitive microbeads to measure absolute oxygen concentrations in cellular aggregates. Stable microbead integration, a prerequisite for their practical application, was ensured by a site-specific delivery method that is based on the interactions between streptavidin and biotin. The spatial stability introduced by this method allowed for long term measurements of oxygen tension without interfering with the cell aggregation process. By making multiple calibration experiments we further demonstrated the potential of these sensors to measure local oxygen tension in optically dense cellular environments.
巻・号 34(4)
ページ 922-9
公開日 2013-1-1
DOI 10.1016/j.biomaterials.2012.10.019
PII S0142-9612(12)01140-4
PMID 23122803
MeSH Animals Biological Assay / methods* Cell Aggregation / physiology Cell Line Chondrocytes / cytology Chondrocytes / metabolism* Mice Microspheres Molecular Imaging / methods* Oximetry / methods* Oxygen / analysis* Oxygen Consumption / physiology* Spectrometry, Fluorescence / methods*
IF 10.317
引用数 20
WOS 分野 ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)