RRC ID 44154
Author Murakami H, Tanabe J, Tamasawa N, Matsumura K, Yamashita M, Matsuki K, Murakami H, Matsui J, Suda T.
Title Reduction of paraoxonase-1 activity may contribute the qualitative impairment of HDL particles in patients with type 2 diabetes.
Journal Diabetes Res. Clin. Pract.
Abstract AIMS:Cholesterol efflux with high-density lipoprotein (HDL) particles has an important role in the first step of reverse cholesterol transport (RCT). However, HDL function in type 2 diabetes has not been well investigated thoroughly. We measured cholesterol efflux in 36 patients with type 2 diabetes compared with 9 controls without diabetes.
METHODS:The HDL fraction was separated with polyacrylamide gel and recovered using the protein recovery system. Concentration adjusted HDL fraction was used to determine HDL-mediated cholesterol efflux (Efflux-hdl) from THP-1 derived macrophages. We measured paraoxonase-1 (PON 1) activity to determine antioxidation capacity, serum amyloid A protein (SAA) to determine inflammatory response, and carboxymethyl-lysin (CML) to determine antiglucoxidative capacity.
RESULTS:Efflux-hdl demonstrated no correlation with plasma apoprotein A-1 (ApoA-I) or HDL-cholesterol in patients with diabetes. PON1 activity in the patients' HDL fraction was positively correlated with Efflux-hdl (r=0.39, p=0.02), and showed a negative tendency with HbA1c levels (r=-0.28, p=0.10). SAA and CML levels did not demonstrate correlation with Efflux-hdl in patients with diabetes.
CONCLUSION:We confirmed the functional changes in HDL particles in the patients. Efflux-hdl from macrophages was reduced depending upon the decrease in PON1 activity, which was inversely related to HbA1c levels.
Volume 99(1)
Pages 30-8
Published 2013-1
DOI 10.1016/j.diabres.2012.10.022
PII S0168-8227(12)00389-0
PMID 23137417
MeSH Aged Apolipoprotein A-I / blood Apolipoprotein A-I / metabolism Aryldialkylphosphatase / blood* Atherosclerosis / etiology Atherosclerosis / metabolism Biological Transport Biomarkers / blood Biomarkers / metabolism Cell Line Cholesterol / blood Cholesterol / metabolism Cholesterol, HDL / blood Cholesterol, HDL / metabolism Diabetes Mellitus, Type 2 / immunology Diabetes Mellitus, Type 2 / metabolism* Diabetes Mellitus, Type 2 / physiopathology Female Glycated Hemoglobin A / analysis Humans Hyperglycemia / etiology Hyperglycemia / metabolism Lipoproteins, HDL / blood* Lipoproteins, HDL / isolation & purification Lipoproteins, HDL / metabolism Lysine / analogs & derivatives Lysine / blood Lysine / metabolism Macrophages / metabolism Male Middle Aged Serum Amyloid A Protein / analysis Serum Amyloid A Protein / metabolism
IF 2.548
Times Cited 8
Human and Animal Cells