RRC ID 44156
著者 Inoue T, Hagiyama M, Enoki E, Sakurai MA, Tan A, Wakayama T, Iseki S, Murakami Y, Fukuda K, Hamanishi C, Ito A.
タイトル Cell adhesion molecule 1 is a new osteoblastic cell adhesion molecule and a diagnostic marker for osteosarcoma.
ジャーナル Life Sci
Abstract AIMS:An immunohistochemical screen for mouse embryos showed that cell adhesion molecule 1 (CADM1), which is an immunoglobulin superfamily member, was expressed in developing bones. Here, we determined the cell types expressing CADM1 and examined its usefulness in the differential diagnosis of osteosarcoma.
MAIN METHODS:Serial sections of murine developing mandibles were stained with anti-CADM1 antibody, by a coloring substrate reactive to alkaline phosphatase (ALP), a broad osteoblastic marker for preosteoblasts to osteoblasts, and by in situ hybridization for osteopontin (OPN), a marker for mature osteoblasts. CADM1 immunohistochemistry was also performed on human remodeling bones, osteosarcomas and other soft tissue tumors.
KEY FINDINGS:CADM1 immunohistochemistry for the mandible revealed that morphologically identifiable osteoblasts expressed CADM1 on their plasma membranes, but neither osteocytes nor bone lining cells did. At the mandibular margin, not only OPN-positive cells but also OPN-negative, ALP-positive cells were CADM1-positive, whereas inside the mandible, OPN-positive cells were often CADM1-negative. Clear membranous staining was detected in the majority of osteosarcomas (46/57), whereas only 13% (6/46) of the other soft tissue tumors were CADM1-positive (P<0.001).
SIGNIFICANCE:These results indicated that CADM1 was a novel osteoblastic adhesion molecule that is expressed transiently during osteoblastic maturation, and a useful diagnostic marker for osteosarcoma cells.
巻・号 92(1)
ページ 91-9
公開日 2013-1-17
DOI 10.1016/j.lfs.2012.10.021
PII S0024-3205(12)00648-0
PMID 23142238
MeSH Adolescent Adult Aged Aged, 80 and over Animals Biomarkers, Tumor / metabolism* Bone Neoplasms / diagnosis* Bone Neoplasms / pathology Cell Adhesion Molecule-1 Cell Adhesion Molecules / metabolism* Cell Membrane / metabolism Child Diagnosis, Differential Female Humans Immunoglobulins / metabolism* Male Mandible / cytology Mandible / metabolism Mice Mice, Inbred C57BL Middle Aged Neoplasms / diagnosis Neoplasms / pathology Osteoblasts / metabolism Osteocytes / metabolism Osteopontin / metabolism Osteosarcoma / diagnosis* Osteosarcoma / pathology Young Adult
IF 3.647
引用数 13
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 HOS(RCB0992) Saos-2(RCB0428)