論文 - 詳細
| RRC ID | 44187 |
|---|---|
| 著者 | Yamamoto A, Kasamatsu A, Ishige S, Koike K, Saito K, Kouzu Y, Koike H, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K. |
| タイトル | Exocyst complex component Sec8: a presumed component in the progression of human oral squamous-cell carcinoma by secretion of matrix metalloproteinases. |
| ジャーナル | J Cancer Res Clin Oncol |
| Abstract |
PURPOSE:Sec8, a component of the exocyst complex, has been implicated in tethering of secretory vesicles to specific regions on the plasma membrane. To investigate the involvement of Sec8 in oral squamous-cell carcinoma (OSCC), we evaluated the expression status and effect of Sec8 in OSCC cell lines. METHODS:Sec8 mRNA and protein expressions in human OSCC cell lines were assessed by quantitative reverse transcriptase-polymerase chain reaction and immunoblotting. Functional analyses, proliferation assay, invasiveness assay, and gelatin zymography in Sec8 knockdown cells were performed. Also the correlation between Sec8 expression and the clinicopathological features in 98 primary OSCCs samples was evaluated by immunohistochemistry. RESULTS:Sec8 mRNA and protein expression were significantly up-regulated in all cell lines (p < 0.05). Sec8 knockdown cells were characterized by reduced cellular proliferation, invasiveness, and secretion of matrix metalloproteinases (MMPs) (MMP-2, proMMP-2, and proMMP-9). Sec8 protein expression in primary OSCCs also was significantly (p < 0.05) greater than in normal counterparts, and higher Sec8 expression was correlated with tumor size (p = 0.03). CONCLUSIONS:Our results suggested for the first time that Sec8 might play a specific role in OSCC progression by mediating MMP secretion. |
| 巻・号 | 139(4) |
| ページ | 533-42 |
| 公開日 | 2013-4-1 |
| DOI | 10.1007/s00432-012-1356-2 |
| PMID | 23207790 |
| MeSH | Adult Aged Aged, 80 and over Apoptosis Blotting, Western Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism* Carcinoma, Squamous Cell / pathology Cell Adhesion Cell Movement Cell Proliferation Disease Progression Female Humans Immunoenzyme Techniques Lymphatic Metastasis Male Matrix Metalloproteinase 2 / genetics Matrix Metalloproteinase 2 / metabolism* Matrix Metalloproteinase 9 / genetics Matrix Metalloproteinase 9 / metabolism* Middle Aged Mouth Mucosa / metabolism* Mouth Neoplasms / genetics Mouth Neoplasms / metabolism* Mouth Neoplasms / pathology RNA, Messenger / genetics RNA, Small Interfering / genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured Vesicular Transport Proteins / antagonists & inhibitors Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* |
| IF | 3.656 |
| 引用数 | 17 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |