論文 - 詳細
| RRC ID | 44194 |
|---|---|
| 著者 | Matsumoto S, Hayashi M, Suzuki Y, Suzuki N, Maeno M, Ogiso B. |
| タイトル | Calcium ions released from mineral trioxide aggregate convert the differentiation pathway of C2C12 cells into osteoblast lineage. |
| ジャーナル | J Endod |
| Abstract |
INTRODUCTION:The purpose of this study was to examine the effect of mineral trioxide aggregate (MTA) on pluripotent-mesenchymal cell differentiation. METHODS:The pluripotent-mesenchymal cell line C2C12 was cultured in a 5% serum medium to induce cell differentiation with or without MTA. The differentiation to myoblasts was analyzed by the immunocytochemical staining of myosin heavy chains. The cellular phenotype-specific markers characterizing the osteoblasts (Runx2 and osterix), chondroblasts (Sox9), myoblasts (MyoD), and adipocytes (LPL) were estimated with mRNA and protein levels by using real-time polymerase chain reaction and Western blot analysis, respectively. To verify that the effect of MTA was caused by the released calcium ions, the mRNA levels were analyzed in the presence or absence of MTA with ethylene glycol tetraacetic acid, calcium chloride, or verapamil. RESULTS:C2C12 cells cultured without MTA altered their phenotype to myoblasts, exhibiting positive reactions to myosin heavy chains. However, the cells cultured with MTA were strongly inhibited from developing into myoblasts. The mRNA and protein expressions of Runx2, osterix, and Sox9 significantly increased with MTA; the expressions of MyoD and LPL decreased significantly. Calcium chloride addition without MTA presented a significant increase of mRNA levels of Runx2, osterix, and Sox9; ethylene glycol tetraacetic acid addition with MTA presented a significant increase of mRNA levels of MyoD and LPL. Verapamil blocked the stimulating or suppressing effect of MTA on these transcription factors. CONCLUSIONS:Our study showed that MTA converted the differentiation pathway of C2C12 cells into osteoblast and/or chondroblast lineages as a result of elution components such as calcium ions from MTA. |
| 巻・号 | 39(1) |
| ページ | 68-75 |
| 公開日 | 2013-1-1 |
| DOI | 10.1016/j.joen.2012.10.006 |
| PII | S0099-2399(12)00939-9 |
| PMID | 23228260 |
| MeSH | Adipocytes / drug effects Aluminum Compounds / chemistry* Calcium / chemistry Calcium / pharmacology* Calcium Channel Blockers / pharmacology Calcium Chloride / pharmacology Calcium Compounds / chemistry* Cell Count Cell Culture Techniques Cell Differentiation / drug effects Cell Line Cell Lineage / drug effects Cell Proliferation / drug effects Chelating Agents / pharmacology Chondrocytes / drug effects Core Binding Factor Alpha 1 Subunit / analysis Drug Combinations Egtazic Acid / pharmacology Humans Lipoprotein Lipase / analysis Mesenchymal Stem Cells / drug effects* Muscle Fibers, Skeletal / drug effects MyoD Protein / analysis Myoblasts / drug effects Myosin Heavy Chains / analysis Osteoblasts / drug effects Oxides / chemistry* Pluripotent Stem Cells / drug effects* Root Canal Filling Materials / chemistry* SOX9 Transcription Factor / analysis Silicates / chemistry* Sp7 Transcription Factor Transcription Factors / analysis Verapamil / pharmacology |
| IF | 3.118 |
| 引用数 | 35 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |