論文 - 詳細
| RRC ID | 44197 |
|---|---|
| 著者 | Itoh T, Miyake Y, Onda A, Kubo J, Ando M, Tsukamasa Y, Takahata M. |
| タイトル | Immunomodulatory effects of heat-killed Enterococcus faecalis TH10 on murine macrophage cells. |
| ジャーナル | Microbiologyopen |
| Abstract |
The objective of this study was to investigate the immunomodulatory effects of heat-killed Enterococcus faecalis TH10 (hk-TH10) and its signal transduction on murine macrophage RAW264 cells. RAW264 cells produced nitric oxide (NO) following hk-TH10 treatment. In order to investigate the mechanisms underlying hk-TH10-stimulated NO production, we further measured NO production in RAW264 cells treated with Toll-like receptor (TLR) 4 inhibitor peptide, NF-κB inhibitor, TLR1-siRNA, TLR2-siRNA, and TLR-6 siRNA. Furthermore, the activation of TLR2-TLR1/6 pathway molecules was analyzed by Western blotting. The result of this study showed that hk-TH10 stimulates NO in RAW264 cells through the activation of the TLR2-TLR1/6 pathway. From our findings, we can conclude that hk-TH10 isolated from a traditional side-dish fermented food (tempeh) may facilitate host immunomodulation. |
| 巻・号 | 1(4) |
| ページ | 373-80 |
| 公開日 | 2012-12-1 |
| DOI | 10.1002/mbo3.41 |
| PMID | 23233442 |
| PMC | PMC3535383 |
| MeSH | Animals Blotting, Western Cell Line Enterococcus faecalis / immunology* Macrophage Activation / immunology Macrophages / immunology* Macrophages / microbiology* Mice NF-kappa B / immunology Nitric Oxide / analysis Nitric Oxide / immunology RNA, Small Interfering / administration & dosage Signal Transduction Toll-Like Receptors / immunology |
| IF | 3.142 |
| 引用数 | 5 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |