RRC ID 44197
著者 Itoh T, Miyake Y, Onda A, Kubo J, Ando M, Tsukamasa Y, Takahata M.
タイトル Immunomodulatory effects of heat-killed Enterococcus faecalis TH10 on murine macrophage cells.
ジャーナル Microbiologyopen
Abstract The objective of this study was to investigate the immunomodulatory effects of heat-killed Enterococcus faecalis TH10 (hk-TH10) and its signal transduction on murine macrophage RAW264 cells. RAW264 cells produced nitric oxide (NO) following hk-TH10 treatment. In order to investigate the mechanisms underlying hk-TH10-stimulated NO production, we further measured NO production in RAW264 cells treated with Toll-like receptor (TLR) 4 inhibitor peptide, NF-κB inhibitor, TLR1-siRNA, TLR2-siRNA, and TLR-6 siRNA. Furthermore, the activation of TLR2-TLR1/6 pathway molecules was analyzed by Western blotting. The result of this study showed that hk-TH10 stimulates NO in RAW264 cells through the activation of the TLR2-TLR1/6 pathway. From our findings, we can conclude that hk-TH10 isolated from a traditional side-dish fermented food (tempeh) may facilitate host immunomodulation.
巻・号 1(4)
ページ 373-80
公開日 2012-12-1
DOI 10.1002/mbo3.41
PMID 23233442
PMC PMC3535383
MeSH Animals Blotting, Western Cell Line Enterococcus faecalis / immunology* Macrophage Activation / immunology Macrophages / immunology* Macrophages / microbiology* Mice NF-kappa B / immunology Nitric Oxide / analysis Nitric Oxide / immunology RNA, Small Interfering / administration & dosage Signal Transduction Toll-Like Receptors / immunology
IF 3.142
引用数 5
WOS 分野 MICROBIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)