Reference - Detail
|Author||Uchakina ON, Castillejo CM, Bridges CC, McKallip RJ.|
|Title||The role of hyaluronic acid in SEB-induced acute lung inflammation.|
We investigated the role of the extracellular matrix component, hyaluronic acid (HA) in SEB-induced ALI/ARDS. Intranasal exposure of mice to SEB led to a significant increase in the level of soluble hyaluronic acid in the lungs. Similarly, in an endothelial cell/spleen cell co-culture, SEB exposure led to significant increases in soluble levels of hyaluronic acid, cellular proliferation, and cytokine production compared with SEB-exposed spleen cells or endothelial cells alone. Exposure of SEB-activated spleen cells to hyaluronic acid led to increased cellular proliferation and increased cytokine production. SEB-induced cytokine production and proliferation in vitro were significantly reduced by the hyaluronic acid blocking peptide, Pep-1. Finally, treatment of SEB-exposed mice with Pep-1 significantly reduced SEB-induced ALI/ARDS, through reduction of cytokine production and numbers of lung inflammatory cells, compared to mice treated with a control peptide. Together, these results suggest the possibility of targeting HA for the treatment of SEB-induced ALI/ARDS.
|MeSH||Acute Disease Animals Bronchoalveolar Lavage Fluid / chemistry Bronchoalveolar Lavage Fluid / immunology Cell Line Cell Proliferation / drug effects Cells, Cultured Coculture Techniques Cytokines / genetics Cytokines / immunology Cytokines / metabolism Endothelial Cells / drug effects Endothelial Cells / immunology Endothelial Cells / metabolism Enterotoxins / immunology* Enterotoxins / toxicity Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Glucuronosyltransferase / genetics Glucuronosyltransferase / immunology Glucuronosyltransferase / metabolism Hyaluronan Synthases Hyaluronic Acid / immunology* Hyaluronic Acid / metabolism Hyaluronoglucosaminidase / genetics Hyaluronoglucosaminidase / immunology Hyaluronoglucosaminidase / metabolism Inflammation Mediators / immunology Inflammation Mediators / metabolism Lung / drug effects Lung / immunology* Lung / metabolism Mice Mice, Inbred C57BL Peptides / pharmacology Pneumonia / chemically induced Pneumonia / immunology* Pneumonia / metabolism Reverse Transcriptase Polymerase Chain Reaction Spleen / cytology Spleen / immunology Spleen / metabolism|
|Human and Animal Cells|