| RRC ID |
44210
|
| 著者 |
Futamura Y, Kawatani M, Kazami S, Tanaka K, Muroi M, Shimizu T, Tomita K, Watanabe N, Osada H.
|
| タイトル |
Morphobase, an encyclopedic cell morphology database, and its use for drug target identification.
|
| ジャーナル |
Chem Biol
|
| Abstract |
Visual observation is a powerful approach for screening bioactive compounds that can facilitate the discovery of attractive druggable targets following their chemicobiological validation. So far, many high-content approaches, using sophisticated imaging technology and bioinformatics, have been developed. In our study, we aimed to develop a simpler method that focuses on intact cell images because we found that dynamic changes in morphology are informative, often reflecting the mechanism of action of a drug. Here, we constructed a chemical-genetic phenotype profiling system, based on the high-content cell morphology database Morphobase. This database compiles the phenotypes of cancer cell lines that are induced by hundreds of reference compounds, wherein those of well-characterized anticancer drugs are classified by mode of action. Furthermore, we demonstrate the applicability of this system in identifying NPD6689, NPD8617, and NPD8969 as tubulin inhibitors.
|
| 巻・号 |
19(12)
|
| ページ |
1620-30
|
| 公開日 |
2012-12-21
|
| DOI |
10.1016/j.chembiol.2012.10.014
|
| PII |
S1074-5521(12)00415-2
|
| PMID |
23261605
|
| MeSH |
Animals
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology*
Cell Line, Tumor
Cytological Techniques / methods
Databases, Factual*
Drug Discovery / methods*
Humans
Image Processing, Computer-Assisted / methods
Neoplasms / drug therapy*
Neoplasms / pathology*
Small Molecule Libraries / chemistry
Small Molecule Libraries / pharmacology*
|
| IF |
5.915
|
| 引用数 |
56
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NRK(RCB0043) |
